Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoretic Expression of Serologic Proteins from Sera of Patients with Gastric Cancer

Gastric Cancer Biomarkers

Abstract

There are different proteins with different concentrations present in human serum, post-translational modifications or aberrant expression of these proteins are reported as signs which indicate the process of carcinogenesis in human body. Gastric cancer (GC) ranks second in causing death and fourth in prevalence between all cancers, globally. In recent studies, GC accounts for 8% of the total cancer cases, 10% of the deaths for all cancers and 70% of the cases occurring in developing countries.  The aim of the present study was to profile the differentially expressed serologic proteins using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. In this study fifty serum samples of gastric cancer patients and ten healthy donors were screened by SDS PAGE to estimate the expression of proteins and to compare the up regulation of these proteins with the healthy donors used as control. The results showed that three different proteins were differentially expressed in GC patients while comparing with healthy donors used as control which can be further used as biomarkers to diagnose gastric cancer in its early stages.