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Abstract
Introduction: Berberine, a naturally occurring compound found in several plants such as Berberis vulgaris (barberry), Berberis aristata (tree turmeric), and Phellodendron amurense (Amur cork tree), is a bioactive alkaloid with a long history of use in traditional Chinese and Ayurvedic medicine. Renowned for its broad spectrum of pharmacological properties, berberine is particularly celebrated for its potent anti-hyperglycemic, anti-inflammatory, and antimicrobial effects. It has been reported to have anti-oxidative potentials, scavenging free radicals and reducing oxidative stress, which may contribute to it therapeutic benefits in promoting health and well-being. It helps protect cells from damage, supporting various physiological processes and potentially mitigating the risk of chronic disease.
Objective: This study aims to investigate the antioxidative potential of berberine in edema-induced rat models, and understanding to its therapeutic applications.
Material and Methods: After study ERC approval (ERC/359/01/2023) Healthy male Sprague-Dawley rats (n=25) 7 weeks old were used in experiments performed inter-collaboratively at various affiliated Laboratories. All rats were divided into 5 groups containing 6 rats per group. Group I (Negative Control Group, Non-edema physiological rats), Group II (Positive Control Group, edema induced rats) III, IV and V (edema induced rats with Berberine extract treatment). Except for control of non-induced rats (Group I), edema was induced in rat's paws in groups II, III, IV and V by carrageenan. Following edema induction group III, IV and V were administered the crude powder extract of Berberine 1.9 g, 3.8 g and 5.7 g respectively by mixing in diet, rats were given a treatment of Berberine for the period of 24 days. Whole leaf aqueous extracts at various concentrations (100, 200, 400, and 600 mg/kg of body weight) were given to check the anti-inflammatory potential of Berberine. After treatment animals were sacrificed and samples were collected to analyze immunity and oxidative stress. Total Anti-oxidant capacity with stress biomarkers was analyzed. Oxidative stress parameters were measured spectrophotometrically by prescribed colorimetric assay. Data was subjected to ANOVA (two-way) using the statistical package SPSS-22.0.
Results: Results showed that treatments with Berberine showed highly significant effect on stress biomarkers and Total antioxidant capacity. Leaf extracts significantly reduced the formation of edema induced by carrageenan and formaldehyde and granuloma formation in a dose-dependent manner.
Conclusion: Berberine showed strong anti-oxidative potential with best pharmaceutical significance.
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