Abstract
A field experiment was conducted in the Botanical Garden, University of Balochistan, Quetta, Balochistan, Pakistan, to assess the tolerance potential of two lentil (Lens culinaris Medik.) varieties, i.e., Dasht-21 and Black-Panjgur, under single and combined chromium and salinity stress with exogenous supplementation of Salicylic acid (SA). Design of the experiment was RCBD under factorial with 3 replicates. After 25 days of germination, stress (2 levels of Chromium and salinity) was applied, and after one week of stress, foliar supplementation of SA was applied. Plants per treatment were harvested after 10 days of foliar supplementation to analyse the nutritional status of lentil varieties. Results revealed that both varieties exhibited high levels of sodium (Na+) and Chromium (Cr) under single and combined stress, while SA ameliorated the accumulation of Na (20-25%) and Cr (15-20%) in root and shoots of lentils. Considering the beneficial minerals, i.e., potassium (K) and magnesium (Mg), it was observed that the plants treated with SA had the maximum concentration of K (15-35%) and Mg (20-30%) in plant tissues even under stress, while K and Mg concentrations were hampered in plants without SA supplementation under stress. Thus, the present study recommends foliar application of SA to alleviate the impacts of salinity and chromium stress by balancing the nutrient status of plants.
References
References:
1. Sharafi S, Salehi F. Comprehensive assessment of heavy metal (HMs) contamination and associated health risks in agricultural soils and groundwater proximal to industrial sites. Scientific Reports. 2025;15(1):7518.
2. Kumar R, Ivy N, Bhattacharya S, Dey A, Sharma P. Coupled effects of microplastics and heavy metals on plants: Uptake, bioaccumulation, and environmental health perspectives. Science of the Total Environment. 2022;836:155619.
3. Sahito ZA, Zehra A, Yu S, Chen S, Arif MAR, Raza ST. Folic acid supplementation improves seed germination, seedling growth and cadmium uptake in a mining ecotype of Solanum nigrum L. Environmental Technology & Innovation. 2024;34:103600.
4. Abd Elnabi MK, Elkaliny NE, Elyazied MM, Azab SH, Elkhalifa SA, Elmasry S. Toxicity of heavy metals and recent advances in their removal: a review. Toxics. 2023;11(7):580.
5. Xu W, Jin Y, Zeng G. Introduction of heavy metals contamination in the water and soil: a review on source, toxicity and remediation methods. Green Chemistry Letters and Reviews. 2024;17(1):2404235.
6. Zulfiqar U, Haider FU, Ahmad M, Hussain S, Maqsood MF, Ishfaq M. Chromium toxicity, speciation, and remediation strategies in soil-plant interface: A critical review. Frontiers in Plant Science. 2023;13:1081624.
7. Sharma A, Kapoor D, Wang J, Shahzad B, Kumar V, Bali AS. Chromium bioaccumulation and its impacts on plants: an overview. Plants. 2020;9(1):100.
8. Abdelhamid MT, El-Masry RR, Darwish DS, Abdalla MM, Oba S, Ragab R. Mechanisms of seed priming involved in salt stress amelioration. Priming and pretreatment of seeds and seedlings: Implication in plant stress tolerance and enhancing productivity in crop plants: Springer; 2019. p. 219-51.
9. El Sabagh A, Hossain A, Barutçular C, Iqbal MA, Islam MS, Fahad S. Consequences of salinity stress on the quality of crops and its mitigation strategies for sustainable crop production: an outlook of arid and semi-arid regions. Environment, climate, plant and vegetation growth: Springer; 2020. p. 503-33.
10. Islam MS, Akhter MM, El Sabagh A, Liu LY, Nguyen NT, Ueda A. Comparative studies on growth and physiological responses to saline and alkaline stresses of Foxtail millet ('Setaria italica'L.) and Proso millet ('Panicum miliaceum'L.). Australian Journal of Crop Science. 2011;5(10):1269-77.
11. Yang Y, Guo Y. Unraveling salt stress signaling in plants. Journal of integrative plant biology. 2018;60(9):796-804.
12. Morari F, Meggio F, Lunardon A, Scudiero E, Forestan C, Farinati S, Varotto S. Time course of biochemical, physiological, and molecular responses to field-mimicked conditions of drought, salinity, and recovery in two maize lines. Frontiers in Plant Science. 2015;6:314.
13. Monsur MB, Ivy NA, Haque MM, Hasanuzzaman M, El Sabagh A, Rohman MM. Oxidative stress tolerance mechanism in rice under salinity. Phyton. 2020;89(3):497.
14. Ashraf M, Shahzad SM, Imtiaz M, Rizwan MS. Salinity effects on nitrogen metabolism in plants–focusing on the activities of nitrogen metabolizing enzymes: A review. Journal of plant nutrition. 2018;41(8):1065-81.
15. Khan HA, Siddique KH, Munir R, Colmer TD. Salt sensitivity in chickpea: Growth, photosynthesis, seed yield components and tissue ion regulation in contrasting genotypes. Journal of plant physiology. 2015;182:1-12.
16. Liu L, Nakamura Y, Taliman NA, Sabagh AE, Moghaieb RE, Saneoka H. Differences in the growth and physiological responses of the leaves of Peucedanum japonicum and Hordeum vulgare exposed to salinity. Agriculture. 2020;10(8):317.
17. Ghassemi-Golezani K, Hosseinzadeh-Mahootchi A. Improving physiological performance of safflower under salt stress by application of salicylic acid and jasmonic acid. WALIA J. 2015;31(S1):104-9.
18. Delavari P, Baghizadeh A, Enteshari S, Kalantari KM, Yazdanpanah A, Mousavi E. The effects of salicylic acid on some of biochemical and morphological characteristic of Ocimum basilicucm under salinity stress. 2010.
19. Yazdanpanah S, Baghizadeh A, Abbassi F. The interaction between drought stress and salicylic and ascorbic acids on some biochemical characteristics of Satureja hortensis. African Journal of Agricultural Research. 2011;6(4):798-807.
20. Arfan M, Athar HR, Ashraf M. Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress? Journal of plant physiology. 2007;164(6):685-94.
21. Wildermuth MC, Dewdney J, Wu G, Ausubel FM. Isochorismate synthase is required to synthesize salicylic acid for plant defence. Nature. 2001;414(6863):562-5.
22. Zhang S, Tan X, Zhou Y, Liu N. Effects of a heavy metal (cadmium) on the responses of subtropical coastal tree species to drought stress. Environmental Science and Pollution Research. 2023;30(5):12682-94.
23. Song W, Shao H, Zheng A, Zhao L, Xu Y. Advances in roles of salicylic acid in plant tolerance responses to biotic and abiotic stresses. Plants. 2023;12(19):3475.
24. Li X-C, Chang C, Pei Z-M. Reactive oxygen species in drought-induced stomatal closure: the potential roles of NPR1. Plants. 2023;12(18):3194.
25. Sharma A, Sidhu GPS, Araniti F, Bali AS, Shahzad B, Tripathi DK. The role of salicylic acid in plants exposed to heavy metals. Molecules. 2020;25(3):540.
26. Saleem M, Fariduddin Q, Castroverde CDM. Salicylic acid: A key regulator of redox signalling and plant immunity. Plant Physiology and Biochemistry. 2021;168:381-97.
27. Kaya C, Ugurlar F, Ashraf M, Ahmad P. Salicylic acid interacts with other plant growth regulators and signal molecules in response to stressful environments in plants. Plant Physiology and Biochemistry. 2023;196:431-43.
28. Torun H, Cetin B, Stojnic S, Petrík P. Salicylic acid alleviates the effects of cadmium and drought stress by regulating water status, ions, and antioxidant defense in Pterocarya fraxinifolia. Frontiers in Plant Science. 2024;14:1339201.
29. Tripti, Kumar A, Maleva M, Borisova G, Rajkumar M. Amaranthus biochar-based microbial cell composites for alleviation of drought and cadmium stress: A novel bioremediation approach. Plants. 2023;12(10):1973.
30. Arumuganathan K, Earle E. Nuclear DNA content of some important plant species. Plant molecular biology reporter. 1991;9(3):208-18.
31. Ayub K, Rahim M, Khan A. Performance of exotic lentil varieties under rainfed conditions in Mingora (NWFP) Pakistan. J Bio Sci. 2001;1:343-4.
32. Rahim S, Dawar S, Tariq M. Mycoflora associated with lentil (Lens culinaris L.) seeds of Pakistan. Pak J Bot. 2010;42(6):4345-52.
33. Sulistiani WS, Widowati H, Sari K, Sutanto A. Effect of chromium metal accumulation on the magnesium absorption and chlorophyll content in vegetables. Makara Journal of Science. 2021;25(1):5.
34. Huda AN, Swaraz A, Reza MA, Haque MA, Kabir AH. Remediation of chromium toxicity through exogenous salicylic acid in rice (Oryza sativa L.). Water, Air, & Soil Pollution. 2016;227(8):278.
35. Gupta S, Seth CS. Salicylic acid alleviates chromium (VI) toxicity by restricting its uptake, improving photosynthesis and augmenting antioxidant defense in Solanum lycopersicum L. Physiology and Molecular Biology of Plants. 2021;27(11):2651-64.
36. Alharbi K, Al-Osaimi AA, Alghamdi BA. Sodium chloride (NaCl)-induced physiological alteration and oxidative stress generation in Pisum sativum (L.): A toxicity assessment. ACS omega. 2022;7(24):20819-32.
37. Ebrahimi R, Bhatla SC. Effect of sodium chloride levels on growth, water status, uptake, transport, and accumulation pattern of sodium and chloride ions in young sunflower plants. Communications in soil science and plant analysis. 2011;42(7):815-31.
38. Bouallegue A, Horchani F, Souissi F, Tebini M, Jalali K, Ahmed HB. Enhancement of plant growth in lentil (Lens culinaris) under salinity stress by exogenous application or seed priming with salicylic acid and hydrogen peroxide. PLoS One. 2025;20(6):e0326093.
39. Sharavdorj K, Byambadorj S-O, Jang Y, Cho J-W. Application of magnesium and calcium sulfate on growth and physiology of forage crops under long-term salinity stress. Plants. 2022;11(24):3576.
40. Hussein M, Rezk A, El-Nasharty A, Mehanna H. Nutritional and growth response of canola plants to salicylic acid under salt stress conditions. Int J ChemTech Res. 2015;8(6):574-81.
41. Talaat NB, Hanafy AM. Spermine-salicylic acid interplay restrains salt toxicity in wheat (Triticum aestivum L.). Plants. 2023a;12(2):352.
42. Talaat NB, Mahmoud AWM, Hanafy AM. Co-application of salicylic acid and spermine alleviates salt stress toxicity in wheat: growth, nutrient acquisition, osmolytes accumulation, and antioxidant response. Acta Physiologiae Plantarum. 2023b;45(1):1.
43. Ali S, Cai S, Zeng F, Qiu B, Zhang G. Effect of salinity and hexavalent chromium stresses on uptake and accumulation of mineral elements in barley genotypes differing in salt tolerance. Journal of Plant Nutrition. 2012;35(6):827-39.
44. Tripathi DK, Singh VP, Kumar D, Chauhan DK. Impact of exogenous silicon addition on chromium uptake, growth, mineral elements, oxidative stress, antioxidant capacity, and leaf and root structures in rice seedlings exposed to hexavalent chromium. Acta physiologiae plantarum. 2012;34(1):279-89.
45. Yildirim E, Turan M, Guvenc I. Effect of foliar salicylic acid applications on growth, chlorophyll, and mineral content of cucumber grown under salt stress. Journal of plant nutrition. 2008;31(3):593-612.
46. Gunes A, Inal A, Alpaslan M, Eraslan F, Bagci EG, Cicek N. Salicylic acid induced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maize (Zea mays L.) grown under salinity. Journal of plant physiology. 2007;164(6):728-36.
47. Gunes A, Inal A, Alpaslan M, Cicek N, Guneri E, Eraslan F, Guzelordu T. Effects of exogenously applied salicylic acid on the induction of multiple stress tolerance and mineral nutrition in maize (Zea mays L.) (Einfluss einer Salicylsäure–Applikation auf die Induktion von Stresstoleranz sowie Nährstoffaufnahme von Mais [Zea mays L.]). Archives of agronomy and soil science. 2005;51(6):687-95.
48. Sarker U, Oba S. The response of salinity stress-induced A. tricolor to growth, anatomy, physiology, non-enzymatic and enzymatic antioxidants. Frontiers in plant science. 2020;11:559876.
49. Azooz M. Salt stress mitigation by seed priming with salicylic acid in two faba bean genotypes differing in salt tolerance. International Journal of Agriculture and Biology. 2009;11(4):343-50.
50. Islam F, Yasmeen T, Arif MS, Riaz M, Shahzad SM, Imran Q, Ali I. Combined ability of chromium (Cr) tolerant plant growth promoting bacteria (PGPB) and salicylic acid (SA) in attenuation of chromium stress in maize plants. Plant Physiology and Biochemistry. 2016;108:456-67.
51. Szepesi Á. Role of salicylic acid pre-treatment on the acclimation of tomato plants to salt-and osmotic stress. Acta Biologica Szegediensis. 2005;49(1-2):123-5.
52. Alghamdi SA, Alharby HF, Abbas G, Al-Solami HM, Younas A, Aldehri M. Salicylic acid-and potassium-enhanced resilience of quinoa (Chenopodium quinoa Willd.) against salinity and cadmium stress through mitigating ionic and oxidative stress. Plants. 2023;12(19):3450.

This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2025 Pak-Euro Journal of Medical and Life Sciences