Physicochemical Properties and Mineral Element Composition of Pomegranate (Punica Granatum L.) from Balochistan, Pakistan: An Atomic Absorption Spectroscopic Sudy

Authors

  • Habib Ullah Department of Chemistry, Faculty of Physical and Environmental Sciences, University of Balochistan, Quetta, Pakistan Author
  • Anum Iqbal Department of Chemistry, Faculty of Physical and Environmental Sciences, University of Balochistan, Quetta, Pakistan Author
  • Saleha Suleman Khan Department of Chemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan Author
  • Irshad Ali Department of Chemistry, Faculty of Physical and Environmental Sciences, University of Balochistan, Quetta, Pakistan Author
  • Nimra Fazal Department of Chemistry, Faculty of Physical and Environmental Sciences, University of Balochistan, Quetta, Pakistan Author
  • Saqiba Tahir Department of Chemistry, Faculty of Physical and Environmental Sciences, University of Balochistan, Quetta, Pakistan Author
  • Kanwal Iqbal Department of Chemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan Author
  • Afroz Rias Department of Botany, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan Author
  • Saima Maher Department of Chemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan Author
  • Raza Ullah H.E.J Research Institute of Chemistry, University of Karachi-75270, Karachi, Pakistan Author

DOI:

https://doi.org/10.31580/pjmls.v8iSp3.3471

Keywords:

Atomic absorption spectroscopy (AAS), Balochistan, Heavy metals, Pomegranate

Abstract

 

Background: The pomegranate (Punica granatum L.) is a significant fruit crop because of its nutritional and health-promoting characteristics. Nonetheless, variations in environmental conditions and the accumulation of heavy metals might have an impact on the quality of the fruit and on food safety. The present study evaluated pomegranate's selected physicochemical properties and heavy metal contents for samples obtained from various areas of Balochistan, Pakistan. Methodology: Khuzdar, Zehri, Baghbana, Zeedi, and Wadh are amongst the various regions of Balochistan where samples were collected. Cadmium (Cd), Copper (Cu), Nickle (Ni), Lead (Pb), and Zinc (Zn) were analyzed using Atomic Absorption Spectroscopy (AAS) after standard sample preparation and acid digestion while Physicochemical properties, including pH, ash content, and titratable acidity, were also determined.

Results: The ash content and titratable acidity ranged from 0.3–0.7% and 0.5–1.5 respectively, while pH ranged from 2.2 to 3.8, confirming the acidic nature of pomegranate. All the metals analyzed were found with a significant level of regional variation. Zinc was the most abundant metal, followed by copper, nickel, lead and cadmium. Khuzdar generally exhibited higher metal concentrations than Wadh, which showed comparatively lower concentrations for most of the analyzed metals. These variations may be due to differences in soil, irrigation water, agricultural methods, and other environmental conditions.

Conclusion: The results revealed the regional differences in the physicochemical and elemental composition of pomegranates from Balochistan. The physicochemical results in general could not be directly related to metal accumulation because region-specific values were not reported; however, the findings highlight the need for regular monitoring of heavy metals in fruits and their growing environments to support food safety and maintain fruit quality.

 

Author Biographies

  • Habib Ullah, Department of Chemistry, Faculty of Physical and Environmental Sciences, University of Balochistan, Quetta, Pakistan

    Faculty of Physical and Environmental Sciences, Department of Chemistry, University of Balochistan, Pakistan.

  • Saqiba Tahir, Department of Chemistry, Faculty of Physical and Environmental Sciences, University of Balochistan, Quetta, Pakistan

    Faculty of Physical and Environmental Sciences, Department of Chemistry, University of Balochistan, Pakistan.

References

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2. Ali HH, Al-Ecehagy JMK, Noori AM. In vitro propagation of pomegranate (Punica granatum L.). In: IOP Conference Series: Earth and Environmental Science. 2023;1252(1):012102.

3. Kshirsagar KR, Pathak SS, Patil SM. Pomegranate (Punica granatum L): a fruitful fountain of remedial potential. Cureus. 2023;15(9).

4. Mohammadi M, Boghrati Z, Emami SA, Akaberi M. Pomegranate: a review of the heavenly healer’s past, present, and future. Iran J Basic Med Sci. 2023;26(11):1245.

5. Andrew OV, Ndiokwere CG. Investigation of natural plant extracts for antimicrobial properties. Acad World J Sci Eng Innov. 2025;2(1).

6. Pant BD, Adhikari S, Shrestha N, Baral J, Paudyal H, Ghimire KN, et al. Iron-loaded Punica granatum peel: an effective biosorbent for the excision of arsenite from water. Heliyon. 2024;10(17).

7. Hellen LE, Christina F, Othman OC. Determination of physico-chemical properties of pomegranate (Punica granatum L.) fruits of Dares Salaam, Tanzania. J Food Nutr Sci. 2014;2(6):277-284.

8. Mditshwa A, Fawole OA, Opara UL, Al-Said F, Al-Yahyai R. Phytochemical content, antioxidant capacity and physicochemical properties of pomegranate grown in different microclimates in South Africa. S Afr J Plant Soil. 2013;30(2):81-90.

9. Roussos PA. Climate change challenges in temperate and sub-tropical fruit tree cultivation. Encyclopedia. 2024;4(1):558-582.

10. Salimi Y, Entezari S, Alimohammadi F, Azimi N, Ahadi M, Heydari A, et al. Uses of pomegranate in dentistry: a comprehensive review of literature. Malays J Med Health Sci. 2024;20(6).

11. Kiran S, Tariq A, Iqbal S, Naseem Z, Siddique W, Jabeen S, et al. Punicalagin, a pomegranate polyphenol sensitizes the activity of antibiotics against three MDR pathogens of the Enterobacteriaceae. BMC Complement Med Ther. 2024;24(1):93.

12. Rodriguez S, Kaur K, Sharma M. Extraction methods and bioactive compounds from pomegranate peels: a comprehensive review for sustainable packaging applications. Food Biomacromol. 2025;2(1):42-68.

13. Maqsood S, Arshad MT, Ikram A, Gnedeka KT. Fruit-based diet and gut health: a review. Food Sci Nutr. 2025;13(5): e70159.

14. Kumar D, Roy S, Babu A, Pandey AK. Harnessing fungal bioagents rich in volatile metabolites for sustainable crop protection: a critical review. J Basic Microbiol. 2025;65(4):e70003.

15. Zehra T, Ahmed S, Zehra S. Review of characteristic components, traditional and pharmacological properties of Punica granatum. RADS J Pharm Pharm Sci. 2019;7(1):39-52.

16. Badshah SM, Sultana N, Al-Rawi MBA, Tariq Z, Lin KS, Hussain A, et al. Phytochemical profiling, biological potential and in silico identification of anticancerous compound from Pakistani pomegranate peel. Sci Rep. 2025;15(1):26689.

17. Stefanou V, Papatheodorou S, Tsakni A, Lougovois V, Talelli A, Panourgias G, et al. Anti-inflammatory properties of pomegranate. Int J Adv Res Microbiol Immunol. 2020;2(1):1-13.

18. Hussein L, Gouda M, Buttar HS. Pomegranate, its components, and modern deliverable formulations as potential botanicals in the prevention and treatment of various cancers. Curr Drug Deliv. 2021;18(10):1391-1405.

19. Mohammadzaheri F, Sheikhhossein F, Hemmati A, Ghoreishy SM, Mohammadi-Sartang M, Shiraseb F, et al. The effect of pomegranate products on glycemic profile in adults: a GRADE-assessed systematic review and dose-response meta-analysis of randomized controlled trials. BMC Nutr. 2025;11(1):168.

20. Ullah J, Ahmad I, Nisar M, Ullah I, Khan A, Moon YS, et al. Phytochemical profiling of wild pomegranate (Punica granatum L.): unveiling antioxidant potential and antibacterial activity. Pak J Bot. 2025;57(4):1301-1309.

21. Martins Almeida NL, Peralta LCF, Lara VS. Antimicrobial therapy in dentistry: an evidence-based review of pomegranate fruit properties. Curr Dent. 2024;4(1): E140524229935.

22. Ibrahim M, Singh H, Fahim M, Khan S, Khan J, Arun JK, et al. Nutraceutical interventions for mitigating skin ageing: analysis of mechanisms and efficacy. Curr Pharm Des. 2025.

23. Rahman AU, Esa M, Panichayupakaranant P. The therapeutic potential of pomegranate in the prevention and management of noncommunicable diseases. Food Funct. 2025.

24. Zheng N, Wang Q, Zhang X, Zheng D, Zhang Z, Zhang S. Population health risk due to dietary intake of heavy metals in the industrial area of Huludao city, China. Sci Total Environ. 2007;387(1-3):96-104.

25. Al-Muslhi ASM, Ali HL. Assessing the impact of pomegranate juice on liver tissue in male rats exposed to a commercial energy drink. J Glob Innov Agric Sci. 2024;12: 417-426.

26. Bhat BA, Almilaibary A, Mir RA, Aljarallah BM, Mir WR, Ahmad F, et al. Natural therapeutics in aid of treating Alzheimer’s disease: a green gateway toward ending quest for treating neurological disorders. Front Neurosci. 2022;16: 884345.

27. El Bohi KM, Abdel-Motal SM, Khalil SR, Abd-Elaal MM, Metwally MM, ELhady WM. The efficiency of pomegranate (Punica granatum) peel ethanolic extract in attenuating vancomycin-triggered liver and kidney tissues injury in rats. Environ Sci Pollut Res. 2021;28(6):7134-7150.

1. Mohan M, Mohanavarshaa CA, Priya D. Review of pharmacological and medicinal uses of Punica granatum. Cureus. 2024;16(10).

2. Ali HH, Al-Ecehagy JMK, Noori AM. In vitro propagation of pomegranate (Punica granatum L.). In: IOP Conference Series: Earth and Environmental Science. 2023;1252(1):012102.

3. Kshirsagar KR, Pathak SS, Patil SM. Pomegranate (Punica granatum L): a fruitful fountain of remedial potential. Cureus. 2023;15(9).

4. Mohammadi M, Boghrati Z, Emami SA, Akaberi M. Pomegranate: a review of the heavenly healer’s past, present, and future. Iran J Basic Med Sci. 2023;26(11):1245.

5. Andrew OV, Ndiokwere CG. Investigation of natural plant extracts for antimicrobial properties. Acad World J Sci Eng Innov. 2025;2(1).

6. Pant BD, Adhikari S, Shrestha N, Baral J, Paudyal H, Ghimire KN, et al. Iron-loaded Punica granatum peel: an effective biosorbent for the excision of arsenite from water. Heliyon. 2024;10(17).

7. Hellen LE, Christina F, Othman OC. Determination of physico-chemical properties of pomegranate (Punica granatum L.) fruits of Dares Salaam, Tanzania. J Food Nutr Sci. 2014;2(6):277-284.

8. Mditshwa A, Fawole OA, Opara UL, Al-Said F, Al-Yahyai R. Phytochemical content, antioxidant capacity and physicochemical properties of pomegranate grown in different microclimates in South Africa. S Afr J Plant Soil. 2013;30(2):81-90.

9. Roussos PA. Climate change challenges in temperate and sub-tropical fruit tree cultivation. Encyclopedia. 2024;4(1):558-582.

10. Salimi Y, Entezari S, Alimohammadi F, Azimi N, Ahadi M, Heydari A, et al. Uses of pomegranate in dentistry: a comprehensive review of literature. Malays J Med Health Sci. 2024;20(6).

11. Kiran S, Tariq A, Iqbal S, Naseem Z, Siddique W, Jabeen S, et al. Punicalagin, a pomegranate polyphenol sensitizes the activity of antibiotics against three MDR pathogens of the Enterobacteriaceae. BMC Complement Med Ther. 2024;24(1):93.

12. Rodriguez S, Kaur K, Sharma M. Extraction methods and bioactive compounds from pomegranate peels: a comprehensive review for sustainable packaging applications. Food Biomacromol. 2025;2(1):42-68.

13. Maqsood S, Arshad MT, Ikram A, Gnedeka KT. Fruit-based diet and gut health: a review. Food Sci Nutr. 2025;13(5): e70159.

14. Kumar D, Roy S, Babu A, Pandey AK. Harnessing fungal bioagents rich in volatile metabolites for sustainable crop protection: a critical review. J Basic Microbiol. 2025;65(4):e70003.

15. Zehra T, Ahmed S, Zehra S. Review of characteristic components, traditional and pharmacological properties of Punica granatum. RADS J Pharm Pharm Sci. 2019;7(1):39-52.

16. Badshah SM, Sultana N, Al-Rawi MBA, Tariq Z, Lin KS, Hussain A, et al. Phytochemical profiling, biological potential and in silico identification of anticancerous compound from Pakistani pomegranate peel. Sci Rep. 2025;15(1):26689.

17. Stefanou V, Papatheodorou S, Tsakni A, Lougovois V, Talelli A, Panourgias G, et al. Anti-inflammatory properties of pomegranate. Int J Adv Res Microbiol Immunol. 2020;2(1):1-13.

18. Hussein L, Gouda M, Buttar HS. Pomegranate, its components, and modern deliverable formulations as potential botanicals in the prevention and treatment of various cancers. Curr Drug Deliv. 2021;18(10):1391-1405.

19. Mohammadzaheri F, Sheikhhossein F, Hemmati A, Ghoreishy SM, Mohammadi-Sartang M, Shiraseb F, et al. The effect of pomegranate products on glycemic profile in adults: a GRADE-assessed systematic review and dose-response meta-analysis of randomized controlled trials. BMC Nutr. 2025;11(1):168.

20. Ullah J, Ahmad I, Nisar M, Ullah I, Khan A, Moon YS, et al. Phytochemical profiling of wild pomegranate (Punica granatum L.): unveiling antioxidant potential and antibacterial activity. Pak J Bot. 2025;57(4):1301-1309.

21. Martins Almeida NL, Peralta LCF, Lara VS. Antimicrobial therapy in dentistry: an evidence-based review of pomegranate fruit properties. Curr Dent. 2024;4(1): E140524229935.

22. Ibrahim M, Singh H, Fahim M, Khan S, Khan J, Arun JK, et al. Nutraceutical interventions for mitigating skin ageing: analysis of mechanisms and efficacy. Curr Pharm Des. 2025.

23. Rahman AU, Esa M, Panichayupakaranant P. The therapeutic potential of pomegranate in the prevention and management of noncommunicable diseases. Food Funct. 2025.

24. Zheng N, Wang Q, Zhang X, Zheng D, Zhang Z, Zhang S. Population health risk due to dietary intake of heavy metals in the industrial area of Huludao city, China. Sci Total Environ. 2007;387(1-3):96-104.

25. Al-Muslhi ASM, Ali HL. Assessing the impact of pomegranate juice on liver tissue in male rats exposed to a commercial energy drink. J Glob Innov Agric Sci. 2024;12: 417-426.

26. Bhat BA, Almilaibary A, Mir RA, Aljarallah BM, Mir WR, Ahmad F, et al. Natural therapeutics in aid of treating Alzheimer’s disease: a green gateway toward ending quest for treating neurological disorders. Front Neurosci. 2022;16: 884345.

27. El Bohi KM, Abdel-Motal SM, Khalil SR, Abd-Elaal MM, Metwally MM, ELhady WM. The efficiency of pomegranate (Punica granatum) peel ethanolic extract in attenuating vancomycin-triggered liver and kidney tissues injury in rats. Environ Sci Pollut Res. 2021;28(6):7134-7150.

1. Mohan M, Mohanavarshaa CA, Priya D. Review of pharmacological and medicinal uses of Punica granatum. Cureus. 2024;16(10).

2. Ali HH, Al-Ecehagy JMK, Noori AM. In vitro propagation of pomegranate (Punica granatum L.). In: IOP Conference Series: Earth and Environmental Science. 2023;1252(1):012102.

3. Kshirsagar KR, Pathak SS, Patil SM. Pomegranate (Punica granatum L): a fruitful fountain of remedial potential. Cureus. 2023;15(9).

4. Mohammadi M, Boghrati Z, Emami SA, Akaberi M. Pomegranate: a review of the heavenly healer’s past, present, and future. Iran J Basic Med Sci. 2023;26(11):1245.

5. Andrew OV, Ndiokwere CG. Investigation of natural plant extracts for antimicrobial properties. Acad World J Sci Eng Innov. 2025;2(1).

6. Pant BD, Adhikari S, Shrestha N, Baral J, Paudyal H, Ghimire KN, et al. Iron-loaded Punica granatum peel: an effective biosorbent for the excision of arsenite from water. Heliyon. 2024;10(17).

7. Hellen LE, Christina F, Othman OC. Determination of physico-chemical properties of pomegranate (Punica granatum L.) fruits of Dares Salaam, Tanzania. J Food Nutr Sci. 2014;2(6):277-284.

8. Mditshwa A, Fawole OA, Opara UL, Al-Said F, Al-Yahyai R. Phytochemical content, antioxidant capacity and physicochemical properties of pomegranate grown in different microclimates in South Africa. S Afr J Plant Soil. 2013;30(2):81-90.

9. Roussos PA. Climate change challenges in temperate and sub-tropical fruit tree cultivation. Encyclopedia. 2024;4(1):558-582.

10. Salimi Y, Entezari S, Alimohammadi F, Azimi N, Ahadi M, Heydari A, et al. Uses of pomegranate in dentistry: a comprehensive review of literature. Malays J Med Health Sci. 2024;20(6).

11. Kiran S, Tariq A, Iqbal S, Naseem Z, Siddique W, Jabeen S, et al. Punicalagin, a pomegranate polyphenol sensitizes the activity of antibiotics against three MDR pathogens of the Enterobacteriaceae. BMC Complement Med Ther. 2024;24(1):93.

12. Rodriguez S, Kaur K, Sharma M. Extraction methods and bioactive compounds from pomegranate peels: a comprehensive review for sustainable packaging applications. Food Biomacromol. 2025;2(1):42-68.

13. Maqsood S, Arshad MT, Ikram A, Gnedeka KT. Fruit-based diet and gut health: a review. Food Sci Nutr. 2025;13(5): e70159.

14. Kumar D, Roy S, Babu A, Pandey AK. Harnessing fungal bioagents rich in volatile metabolites for sustainable crop protection: a critical review. J Basic Microbiol. 2025;65(4):e70003.

15. Zehra T, Ahmed S, Zehra S. Review of characteristic components, traditional and pharmacological properties of Punica granatum. RADS J Pharm Pharm Sci. 2019;7(1):39-52.

16. Badshah SM, Sultana N, Al-Rawi MBA, Tariq Z, Lin KS, Hussain A, et al. Phytochemical profiling, biological potential and in silico identification of anticancerous compound from Pakistani pomegranate peel. Sci Rep. 2025;15(1):26689.

17. Stefanou V, Papatheodorou S, Tsakni A, Lougovois V, Talelli A, Panourgias G, et al. Anti-inflammatory properties of pomegranate. Int J Adv Res Microbiol Immunol. 2020;2(1):1-13.

18. Hussein L, Gouda M, Buttar HS. Pomegranate, its components, and modern deliverable formulations as potential botanicals in the prevention and treatment of various cancers. Curr Drug Deliv. 2021;18(10):1391-1405.

19. Mohammadzaheri F, Sheikhhossein F, Hemmati A, Ghoreishy SM, Mohammadi-Sartang M, Shiraseb F, et al. The effect of pomegranate products on glycemic profile in adults: a GRADE-assessed systematic review and dose-response meta-analysis of randomized controlled trials. BMC Nutr. 2025;11(1):168.

20. Ullah J, Ahmad I, Nisar M, Ullah I, Khan A, Moon YS, et al. Phytochemical profiling of wild pomegranate (Punica granatum L.): unveiling antioxidant potential and antibacterial activity. Pak J Bot. 2025;57(4):1301-1309.

21. Martins Almeida NL, Peralta LCF, Lara VS. Antimicrobial therapy in dentistry: an evidence-based review of pomegranate fruit properties. Curr Dent. 2024;4(1): E140524229935.

22. Ibrahim M, Singh H, Fahim M, Khan S, Khan J, Arun JK, et al. Nutraceutical interventions for mitigating skin ageing: analysis of mechanisms and efficacy. Curr Pharm Des. 2025.

23. Rahman AU, Esa M, Panichayupakaranant P. The therapeutic potential of pomegranate in the prevention and management of noncommunicable diseases. Food Funct. 2025.

24. Zheng N, Wang Q, Zhang X, Zheng D, Zhang Z, Zhang S. Population health risk due to dietary intake of heavy metals in the industrial area of Huludao city, China. Sci Total Environ. 2007;387(1-3):96-104.

25. Al-Muslhi ASM, Ali HL. Assessing the impact of pomegranate juice on liver tissue in male rats exposed to a commercial energy drink. J Glob Innov Agric Sci. 2024;12: 417-426.

26. Bhat BA, Almilaibary A, Mir RA, Aljarallah BM, Mir WR, Ahmad F, et al. Natural therapeutics in aid of treating Alzheimer’s disease: a green gateway toward ending quest for treating neurological disorders. Front Neurosci. 2022;16: 884345.

27. El Bohi KM, Abdel-Motal SM, Khalil SR, Abd-Elaal MM, Metwally MM, ELhady WM. The efficiency of pomegranate (Punica granatum) peel ethanolic extract in attenuating vancomycin-triggered liver and kidney tissues injury in rats. Environ Sci Pollut Res. 2021;28(6):7134-7150.

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Published

2026-03-31

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Research Article

How to Cite

Physicochemical Properties and Mineral Element Composition of Pomegranate (Punica Granatum L.) from Balochistan, Pakistan: An Atomic Absorption Spectroscopic Sudy. (2026). Pak-Euro Journal of Medical and Life Sciences, 8(Special 3), S277-S286. https://doi.org/10.31580/pjmls.v8iSp3.3471

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