Abstract
Milk can serve as a source of bacterial contamination. Being a suitable medium for different bacterial growth, improperly handled sheep milk improperly dealt with using contaminated utensils or dirty hands, can pose serious health hazards. Infectious diseases that occur due to pathogenic bacteria are the most common and widespread in areas where sheep milk is consumed. Some pathogenic bacteria in milk are capable of breaking of important milk components and their utilization, such as lactose, which can disturb the nutritional profile of milk. Disseminated through contaminated sheep milk, some of these pathogens can be life threatening. Therefore, the objective of the study was to assess the prevalence of bacterial contamination and associated factors in the milk collected from different sheep breeds. The five breeds of sheep (Ovis aries) were selected (Dumbi, Kochi, Kooka, Balochi and Mengali) from fifteen locations of Sindh and nine Locations of Balochistan, Pakistan. 2 ml of milk was collected in sterile plastic vials and kept at 2-3˚C during the main lactation period of ewes. The pH of sheep milk ranged from 5.32 to 6.70. Milk of Dumbi and Kochi was observed to be the most contaminated, whereas Mengali’s milk was observed to be the least contaminated. The isolated bacteria belonged to E. coli, Pseudomonas and Listeria species.
References
1. Berhe G, Wasihun AG, Kassaye E, Gebreselasie K. Milk-borne bacterial health hazards in milk produced for commercial purpose in Tigray, northern Ethiopia. BMC Public Health. 2020;20(1):894.
2. Ziad KT, Hayat U, Bacha MS. An economic assessment of problems associated with small-scale farmers in the dairy sector of Pakistan (a case study of Punjab Province). Sarhad J Agric. 2019;35(1):782-7.
3. Khan MS, Khan MALI, Ahmad S, Mahmood S. Genetic resources and diversity in Pakistani sheep. Int J Agric Biol. 2007;6:941-4.
4. Ariyanti T, Rachmawati F, Noor SM. Contamination of Escherichia coli O157:H7 in milk and its dairy products in Depok, Cianjur, Sukabumi and Bandung. IOP Conf Ser Earth Environ Sci. 2022;1107(1):012047.
5. Li X, Zheng J, Zhao W, Wu Y. Prevalence of Listeria monocytogenes in milk and dairy product supply chains: a global systematic review and meta-analysis. Foodborne Pathog Dis. 2024;21(9):526-35.
6. Champagne CP, Laing RR, Roy D, Mafu AA, Griffiths MW, White C. Psychrotrophs in dairy products: their effects and their control. Crit Rev Food Sci Nutr. 1994;34(1):1-30.
7. Bagnoli D, Pannella G, Vergalito F, Coppola F, Messia MC, Succi M. Influence of the Mefite D’Ansanto Valley on the chemical characteristics and microbial consortia of raw sheep milk for the production of Pecorino Carmasciano cheese. Food Biosci. 2025;66:106254.
8. Ruiz MJ, Medina LM, Palacio MI, Vega MF, Etcheverría S, Frizzo LS, Etcheverría AI. Investigation of some probiotic and technological properties of lactic acid bacteria strains isolated from artisanal sheep milk cheese and their growth in goat milk. Small Rumin Res. 2024;238:107329.
9. Zar JH. Biostatistical analysis. New Delhi: Pearson Education India; 1999.
10. Roșu RD, Morar A, Ban-Cucerzan A, Imre M, Sallam KI, Maha AAA, Abd-Elghany SM, Popa SA, Pătrînjan RT, Morar D, Imre K. The microbiological quality of raw ovine milk in the Banat region of Romania with a focus on Escherichia coli and its pathogenic potential and antimicrobial resistance. Vet Sci. 2024;11(11):562.
11. McKusick BC, Thomas DL, Berger YM, Marnet PG. Effect of milking interval on alveolar versus cisternal milk accumulation and milk production and composition in dairy ewes. J Dairy Sci. 2002;85(9):2197-206.
12. Dalgleish DG, Law AJ. pH-induced dissociation of bovine casein micelles. II. Mineral solubilization and its relation to casein release. J Dairy Res. 1989;56(5):727-35.
13. Raynal-Ljutovac K, Lagriffoul G, Paccard P, Guillet I, Chilliard Y. Composition of goat and sheep milk products: An update. Small Rumin Res. 2008;79(1):57–72.
14. Saba CKS, Gonzalez-Zorn B. Microbial contamination of milk and its public health implications. Food Control. 2012;26(2):539–43.
15. Oliver SP, Jayarao BM, Almeida RA. Foodborne pathogens in milk and the dairy farm environment: Food safety and public health implications. Foodborne Pathog Dis. 2005;2(2):115–29.
16. Torkar KG, Teger SG. The microbiological quality of raw milk after introducing the two-day milk collecting system. Acta Agric Slov. 2008;92(1):61–74.
17. Hussein HS, Sakuma T. Prevalence of Shiga toxin-producing Escherichia coli in dairy cattle and their products. J Dairy Sci. 2005;88(2):450–65.
18. Swaminathan B, Gerner-Smidt P. The epidemiology of human listeriosis. Microbes Infect. 2007;9(10):1236–43.
19. Mbindyo CM, Gitao GC, Mulei CM. Udder morphology and its relationship with intramammary infections in dairy goats. Trop Anim Health Prod. 2014;46(1):155–61.
20. Contreras A, Sierra D, Sánchez A, Corrales JC, Marco JC, Paape MJ, Gonzalo C. Mastitis in small ruminants. Small Rumin Res. 2007;68(1–2):145–53.
21. Ledesma-Martínez E, Hernández-Rodríguez C, Olivares-Romero JL, Jiménez-Arellanes A. Influence of pH on milk quality and microbial growth. J Dairy Res. 2018;85(3):340–7.
22. Dalgleish DG, Corredig M. The structure of the casein micelle of milk and its changes during processing. Annu Rev Food Sci Technol. 2012;3:449–67.
23. Decimo M, Morandi S, Silvetti T, Brasca M. Characterization of Gram-negative psychrotrophic bacteria isolated from raw milk and their spoilage potential. Food Microbiol. 2014;39:62–70.
24. EFSA Panel on Biological Hazards. Scientific opinion on raw drinking milk. EFSA J. 2015;13(1):3940.
25. Farrokh C, Jordan K, Auvray F, Glass K, Oppegaard H, Raynaud S. Review of Shiga-toxin producing *Escherichia coli* (STEC) and their public health significance. Foodborne Pathog Dis. 2013;10(6):465–87.
26. Jamali H, Chai LC, Thong KL. Detection and prevalence of antibiotic resistance genes in Listeria monocytogenes from raw milk and dairy products. Food Control. 2013;34(2):121–5.
27. Bălăceanu A, Creangă Ş, Enache M. Microbiological safety of raw cow milk in Banat Region, Romania. Rom Biotechnol Lett. 2016;21(2):11399–407.
28. Mastrorilli E, Rampazzo L, D’Aurizio G. Microbial diversity in sheep milk and cheese production. Int Dairy J. 2018;85:159–68.
29. Khan MT, Zinnah MA, Siddique MP, Rashid MH, Islam MA, Choudhury KA. Physical and microbial quality of raw milk collected from Bangladesh. Int J Dairy Sci. 2008;3(4):158–63.
30. Claeys WL, Cardoen S, Daube G, De Block J, Dewettinck K, Dierick K. Raw or heated cow milk consumption: Review of risks and benefits. Food Control. 2013;31(1):251–62.

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