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Abstract
Plant-mediated synthesis of nanomaterial has been increasingly gaining popularity due to its eco- friendly nature and cost-effectiveness. In the present study, we synthesized silver (Ag) nanoparticles using aqueous extracts of fresh leaves of Ficus carica medicinal plants as bio-reducing agents. Because of the emergence of antibiotic resistance bacteria, our health sector is facing many problems. To overcome these problems, we have tried to synthesize silver nanoparticles fromthe Ficus carica plant due to its antioxidant, anti-inflammatory and antimicrobial properties. This method allowed the synthesis of nanoparticles, which was confirmed by XRD, ultraviolet-visible (UV-Vis) spectrophotometry, SEM and FTIR. UV-Vis spectra and visual observation showed that the color of the fresh leaf extracts turned into grayish brown and brownish yellow, yellow respectively, after treatment with Ag precursors. Moreover, aqueous leaf extracts were separately tested for their antimicrobial activity against Gram-positive S.aureus and Gram-negative Klebsiella bacteria. The results showed that the bacterial growth was inhibited by the extracts containing Ag nanoparticles. Statistical calculation performed using the test showed that zones of inhibition for the two bacteria produced by the aqueous leaf extracts of Ficus carica containing 1mM Ag. There were significant zones of inhibition when using extracts of leaves containing 1mM Ag precursors. It was shown that the aqueous extracts of fresh Ficus carica leaves containing Ag nanoparticles inhibited bacterial growth. In future, we will check the synergistic effect of AgNPs, plant extract and antibiotics.
References
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