Single Nucleotide Polymorphisms Associated with the Meat Production in Balochistani Goat Breeds
Research Article
DOI:
https://doi.org/10.31580/pjmls.v8i1.3185Keywords:
Calpastatin, Crossbreed, Meat Quality, Small Ruminants, SNPsAbstract
Small ruminant farming is a common practice and one of the major sources of livelihood of the rural population of Balochistan. Goat farming has been a common practice throughout the ages for milk, meat, hair, mohair and leather production. Pakistan is the 4th largest goat producing country and Balochistan contributing 22% of the total goat population of the country. Due to its low-fat content, goat meat is healthier compared to beef and mutton. CAST a protein coding gene, has been reported to influence the postmortem tenderization of meat through protein degradation in muscles and considered one of the candidate genes to study meat quality trait in ruminants. The aim of the current study was to investigate CAST gene variants in selected goat breeds under study and its association to meat quality and production and suggest developing plans for future selection of the breeds based on their genetic characterization for the associated traits. The goat breeds of two districts of Balochistan were selected for genetic analysis. The sequencing results revealed 5 single nucleotide polymorphisms including 2 frameshift variations c.290-91delA identified in 70% local and 20% cross breed whereas c.322-25insA at exon 5 identified in 60 % of the local breed, one copy number variant including c.322-25insA and heterozygous missense variation c.326G>T(p.R109I) at exon 5 in 60 % samples of lasi breed and 20% of mixed breed, and 2 splice site variations c.630+13G>A in all breeds under study and c.699-701insG at exon/intron 10 boundary identified in 53% in the local breed.
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