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Abstract
The diverse group of natural compounds known as flavonoids includes subclasses such as flavonols, flavones, flavanones, isoflavones, and anthocyanins, which exhibit extensive therapeutic attributes such as antibacterial, antioxidant, anti-inflammatory, antitumor, as well as neuroprotective effects. Novel flavonoids such as quercetin, kaempferol, naringenin, and genistein, isolated and purified from natural sources have received much notice due to their applications in pharmaceutical and health products. Although conventional extraction and purification methods use organic solvents which put both environment and human health at risk, The resulting need for green technologies that are developed for the extraction and purification of flavonoids has become a critical research objective. This inter-collaborative study between different institutes aimed to explore the application of green technologies for the isolation and purification of novel flavonoids and the determination of their therapeutic activity. Green methodologies utilize eco-friendly solvents and new selective extraction methods like supercritical fluid extraction, ultrasound-assisted extraction, and microwave-assisted extraction. These techniques present many advantages such as a reduction in solvent usage, a shortening the processing time and improving extraction efficiency. In addition, the therapeutic potential of the isolated flavonoids will be determined quantitatively by in vitro as well as in vivo assays such as antioxidant, antibacterial, cytotoxicity, anti-inflammatory and neuroprotective assays. Results from this study will contribute to our understanding of the pharmaceutical potential of novel flavonoids. Finally, this research attempts to set up a green and sustainable approach for the isolation and purification of flavonoids from natural sources. Their therapeutic activities may be valuable in the development of innovative drugs or natural health products. Further, the application of green technologies in the extraction process would provide ecological sustenance, as well as reduce health-related issues of traditional methods.
References
Wang L, Chen M, Lam PY, Dini-Andreote F, Dai L, Wei Z. Multifaceted roles of flavonoids mediating plant-microbe interactions. Microbiome. 2022;10(1):233.
Ku YS, Ng MS, Cheng SS, Lo AW, Xiao Z, Shin TS, Chung G, Lam HM. Understanding the composition, biosynthesis, accumulation and transport of flavonoids in crops for the promotion of crops as healthy sources of flavonoids for human consumption. Nutrients. 2020;12(6):1717.
Chávez-González ML, Sepúlveda L, Verma DK, Luna-García HA, Rodríguez-Durán LV, Ilina A, Aguilar CN. Conventional and emerging extraction processes of flavonoids. Processes. 2020;8(4):434.
Kumar M, Dahuja A, Tiwari S, Punia S, Tak Y, Amarowicz R, Bhoite AG, Singh S, Joshi S, Panesar PS, Saini RP. Recent trends in extraction of plant bioactives using green technologies: A review. Food Chemistry. 2021;353:129431.
Rodríguez De Luna SL, Ramírez-Garza RE, Serna Saldívar SO. Environmentally friendly methods for flavonoid extraction from plant material: Impact of their operating conditions on yield and antioxidant properties. The Scientific World Journal. 2020;2020(1):6792069.
Shrivastav G, Prava Jyoti T, Chandel S, Singh R. Eco-Friendly Extraction: Innovations, Principles, and Comparison with Traditional Methods. Separation & Purification Reviews. 2024:1-7.
Da Silva RF, Carneiro CN, de Sousa CB, Gomez FJ, Espino M, Boiteux J, Fernández MD, Silva MF, Dias FD. Sustainable extraction bioactive compounds procedures in medicinal plants based on the principles of green analytical chemistry: A review. Microchemical Journal. 2022;175:107184.
Slika H, Mansour H, Wehbe N, Nasser SA, Iratni R, Nasrallah G, Shaito A, Ghaddar T, Kobeissy F, Eid AH. Therapeutic potential of flavonoids in cancer: ROS-mediated mechanisms. Biomedicine & Pharmacotherapy. 2022;146:112442.
Schnarr L, Segatto ML, Olsson O, Zuin VG, Kümmerer K. Flavonoids as biopesticides–Systematic assessment of sources, structures, activities and environmental fate. Science of the Total Environment. 2022;824:153781.
Yang L, Gao Y, Bajpai VK, El-Kammar HA, Simal-Gandara J, Cao H, Cheng KW, Wang M, Arroo RR, Zou L, Farag MA. Advance toward isolation, extraction, metabolism and health benefits of kaempferol, a major dietary flavonoid with future perspectives. Critical Reviews in Food Science and Nutrition. 2023;63(16):2773-89.
Chaves JO, De Souza MC, Da Silva LC, Lachos-Perez D, Torres-Mayanga PC, Machado AP, Forster-Carneiro T, Vázquez-Espinosa M, González-de-Peredo AV, Barbero GF, Rostagno MA. Extraction of flavonoids from natural sources using modern techniques. Frontiers in chemistry. 2020;8:507887.
Wang N, Zhu H, Wang M, Zhao S, Sun G, Li Z. Recent advancements in microwave-assisted extraction of flavonoids: a review. Food and Bioprocess Technology. 2024:1-8.
Wang W, Rao L, Wu X, Wang Y, Zhao L, Liao X. Supercritical carbon dioxide applications in food processing. Food Engineering Reviews. 2021:1-22.
Roy A, Khan A, Ahmad I, Alghamdi S, Rajab BS, Babalghith AO, Alshahrani MY, Islam S, Islam MR. Flavonoids a bioactive compound from medicinal plants and its therapeutic applications. BioMed Research International. 2022;2022(1):5445291.
Shen N, Wang T, Gan Q, Liu S, Wang L, Jin B. Plant flavonoids: Classification, distribution, biosynthesis, and antioxidant activity. Food chemistry. 2022;383:132531.
Hasan S, Khatri N, Rahman ZN, Menezes AA, Martini J, Shehjar F, Mujeeb N, Shah ZA. Neuroprotective potential of flavonoids in brain disorders. Brain Sciences. 2023;13(9):1258.
Khan A, Ikram M, Hahm JR, Kim MO. Antioxidant and anti-inflammatory effects of citrus flavonoid hesperetin: Special focus on neurological disorders. Antioxidants. 2020;9(7):609.
Espro C, Paone E, Mauriello F, Gotti R, Uliassi E, Bolognesi ML, Rodríguez-Padrón D, Luque R. Sustainable production of pharmaceutical, nutraceutical and bioactive compounds from biomass and waste. Chemical Society Reviews. 2021;50(20):11191-207.
Ma M, Wang L, Lu X, Wang S, Guo Y, Liang X. One-step fabrication of hydrophobic metal-organic framework@ covalent organic framework hybrid as sorbent for high-performance solid-phase extraction of flavonoids. Journal of Chromatography A. 2023;1691:463814.
Sammani MS, Clavijo S, Cerdà V. Recent, advanced sample pretreatments and analytical methods for flavonoids determination in different samples. TrAC Trends in Analytical Chemistry. 2021;138:116220.
Shamsudin NF, Ahmed QU, Mahmood S, Ali Shah SA, Khatib A, Mukhtar S, Alsharif MA, Parveen H, Zakaria ZA. Antibacterial effects of flavonoids and their structure-activity relationship study: A comparative interpretation. Molecules. 2022;27(4):1149.
Wahnou H, Limami Y, Oudghiri M. Flavonoids and Flavonoid-Based Nanoparticles for Osteoarthritis and Rheumatoid Arthritis Management. BioChem. 2024;4(1):38-61.
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