Aloe's Therapeutic Insights: In Vivo Exploration of Antioxidative Potential in Rat Models
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Introduction: Aloe Vera (A. Vera) is commonly known as “True Aloe” has been reported to have anti-oxidative potentials, scavenging free radicals and reducing oxidative stress, which may contribute to it therapeutic benefits in promoting health and well-being. It helps protect cells from damage, supporting various physiological processes and potentially mitigating the risk of chronic disease.
Objective: This project is designed to probe anti-oxidative potential of A. Vera in edema induced model of rats.
Materials and Methodology: Healthy male Sprague-Dawley rats (n=24) 7 weeks old were used in experiments. All rats were divided into 4 groups containing 6 rats per group. Group I (Control Group, Non-edema physiological rats), Group II (edema induced rats) III and IV (edema induced rats with A. Vera extract treatment). Except control of non-induced rats (Group I), edema was induced in rats paws groups II, III, and IV by carrageenan and formaldehyde. Following edema induction group III and IV were administered with the crude powder extract of A. Vera 1.7 g and 3.4 g respectively by mixing in diet, rats were given treatment of A. Vera for the period of 24 days. Whole leaf aqueous extracts at various concentrations (100, 200, 400, and 600 mg/kg of bw body weight) was given to check anti-inflammatory potential of Aloe Vera. After treatment animals were sacrificed and samples were collected to analyze immunity and oxidative stress. Total Anti-oxidant capacity with stress biomarkers was analyzed. Oxidative stress parameters were measured spectrophotometrically by prescribed colorimetric assay. Data was subjected to ANOVA (two way) using the statistical package SPSS-22.0.
Results: Results showed that treatment with herbal plant have highly significant effect on stress biomarkers and Total anti-oxidant capacity. Leaf extracts significantly reduced formation of edema induced by carrageenan and formaldehyde and granuloma formation in a dose dependent manner.
Conclusion: Aloe Vera showed strong anti-oxidative potential with best pharmaceutical significance.
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