Keywords
amino Acid
buckwheat
HPLC
Baltistan
How to Cite
Abstract
Common buckwheat is grown in high-altitude mountains of Pakistan (Gilgit-Baltistan), is used as a functional food around the world and is considered an important part of the human diet. Matured seeds of twelve (12) local genotypes grown at Khaplu, Ganche, were collected and ground into fine powder after removing hulls. Rutin and amino acid content among these genotypes were determined by employing the High Performance Liquid Chromatography (HPLC) method for rutin and an automatic amino acid analyzer for amino acid. The highest rutin content (32.4 mg/100g) in flour was observed by local genotype BWS-14, followed by genotype BWK-3 (25.3mg/100g), and the lowest rutin content (5.8 mg/100g) was reported by genotype BWSN-05. A significant correlation was observed between genotype BWZ-08 and BWD-1 (r= 0.98) at P< 0.05 for Amino Acid content. Whereas the amino acid content in selected common buckwheat genotypes exhibited a wide variation. The local genotype BWSU-10 and BWA-7 were observed as a good source of lysine and arginine content. Diversity in rutin as well as amino acid contents among native genotypes revealed a potential source of nutraceutical and an important alternative food crop for mountain communities of Baltistan. All these genotypes showed diversity in metabolites, and such varietal differences revealed the worth of conservation of native germplasms in local and national gene banks. Furthermore, this crop can be cultivated at a commercial scale to meet the industrial demand for nutraceutical food in Pakistan.
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