Compositional Evaluation of Fresh Milk Collected from Sale Outlets of Nall, A Remote Area of Balochistan, Pakistan
DOI:
https://doi.org/10.31580/p0fgac71Keywords:
Adulterant screening, Aflatoxin M1, Lacto-scan, Milk composition, Thin layer chromatographyAbstract
Milk is generally considered a complete diet with high nutritional value for all age groups. The present study focused on evaluating the fresh raw milk sold at outlets to determine its physicochemical composition, adulteration, and mycotoxin contamination. The dairy milk samples (n=20) collected from Nall, a nearby remote area of Khuzdar District, were analyzed using a Lacto-scan, Dairy Milk Adulterant Detection Kit, and Thin Layer Chromatography. The results regarding the physicochemical composition, including Total Fats, Solids-Not-Fat (SNF), Density, Sugar Contents (Lactose), Salt, Protein, Added Water, Freezing Point, Conductivity, and pH, showed a significant (P<0.05) difference among sales outlets. The overall mean percent values were 2.85±1.21, 6.31±1.72, 20.37±5.65, 3.43±0.96, 0.50±0.14, 2.32±0.62, 31.65±19.74, -0.39±0.12, 3.67±0.55, and 6.65±0.19, respectively. The results of the screening for adulterants revealed that the collected milk samples were found to be adulterated with detergent, sugar, and skim milk, with adulteration percentages of 10%, 30%, and 25%, respectively. However, adulteration with hydrogen peroxide, boric acid, starch, urea, carbonate, formalin, hypochlorite, sodium chloride, and pulverized soap was not observed. Aflatoxin M1 contamination above the permissible limits was evident in 5% of samples.
References
Poonia A, Jha A, Sharma R, Singh HB, Rai AK, Sharma N. Detection of adulteration in milk: A review. International journal of dairy technology. 2017;70(1):23-42.
Al-Bedrani DI, ALKaisy QH, Rahi AK. Evaluation of milk source on physicochemical, texture, rheological and sensory properties of yogurts. Journal of Applied and Natural Science. 2023;15(1):128.
Ahmad S, Anjum FM, Huma N, Sameen A, Zahoor T. Composition and physico-chemical characteristics of buffalo milk with particular emphasis on lipids, proteins, minerals, enzymes and vitamins. J. Anim. Plant Sci. 2013; (sup I):62-74.
Ahmed M, Khaleeq A, Huma R, Qadir MA, Shafiq MI, Israr A, Ali A, Shahzad S. Optimization and validation procedure for elemental composition of fresh and pasteurized milk in Pakistan employing microwave digestion followed by ICP-OES: A contribution to risk assessment. Food Analytical Methods. 2016;9:2933-42.
Swathi JK, Kauser N. A study on adulteration of milk and milk products from local vendors. International Journal of Biomedical and Advance Research. 2015;6(09):678-81.
Ionescu AD, Cîrîc AI, Begea M. A review of milk frauds and adulterations from a technological perspective. Applied Sciences. 2023 Aug 30;13(17):9821.
Rashid N, Bajwa MA, Rafeeq M, Tariq MM, Abbas F, Awan MA, Khan MA, Shahzad I, Rehman A, Ahmad Z. Prevalence of aflatoxicosis in broiler chickens in Quetta, Pakistan. Pakistan Journal of Zoology. 2013;45(4).
Goncalves L, Dalla Rosa A, Gonzales SL, FELTES MM, Badiale-Furlong E, Dors GC. Incidence of aflatoxin M1 in fresh milk from small farms. Food science and technology. 2017;37(spe):11-5.
Luqman M, Arif A, Zahra N, Wajid U, Abid S. Detection And Removal Of Seasonal Afm1 Occurrence In Various Milk Samples Collected From Different Shops Of Lahore. Chelonian Research Foundation. 2024;19(01):422-33.
Unusan N. Occurrence of aflatoxin M1 in UHT milk in Turkey. Food and Chemical Toxicology. 2006;44(11):1897-900.
Jafari K, Fathabad AE, Fakhri Y, Shamsaei M, Miri M, Farahmandfar R, Khaneghah AM. Aflatoxin M1 in traditional and industrial pasteurized milk samples from Tiran County, Isfahan Province: a probabilistic health risk assessment. Italian Journal of Food Science. 2021;33(SP1):103.
Ferrari L, Fumagalli F, Rizzi N, Grandi E, Vailati S, Manoni M, Ottoboni M, Cheli F, Pinotti L. An eight-year survey on aflatoxin B1 indicates high feed safety in animal feed and forages in northern Italy. Toxins. 2022;14(11):763.
Tadesse S, Berhanu T, Woldegiorgis AZ. Aflatoxin M1 in milk and milk products marketed by local and industrial producers in Bishoftu town of Ethiopia. Food control. 2020;118:107386.
Ferrari L, Rizzi N, Grandi E, Clerici E, Tirloni E, Stella S, Bernardi CE, Pinotti L. Compliance between food and feed safety: Eight-year survey (2013–2021) of aflatoxin M1 in raw milk and aflatoxin B1 in feed in northern Italy. Toxins. 2023;15(3):168.
Rashid N, Bajwa MA, Rafeeq M, Khan MA, Ahmad Z, Tariq MM, Wadood A, Abbas F. Prevalence of aflatoxin B1 in finished commercial broiler feed from west central Pakistan. J. Anim. Plant Sci. 2012;22(1):6-10.
Górska-Warsewicz H, Rejman K, Laskowski W, Czeczotko M. Milk and dairy products and their nutritional contribution to the average polish diet. Nutrients. 2019;11(8):1771.
Yoganandi J, Mehta BM, Wadhwani KN, Darji VB, Aparnathi DK. Comparison of physico-chemical properties of camel milk with cow milk and buffalo milk. Journal of Camel Practice and Research. 2014;21(2):253-8.
Soomro AA, Khaskheli M, Memon MA, Barham GS, Haq IU, Fazlani SN, Khan IA, Lochi GM, Soomro RN. Study on adulteration and composition of milk sold at Badin. Intl J Res Appl Nat Social Sci. 2014;2(9):57-70.
Kausar R, Hameed A, Jamil H, Iqbal Z, Bahadur SU, Jabbar J, Sabir A. Comparative analysis of buffalo and cow milk for quality characteristics and β-N-acetyl-glucosaminidase activity in non-infected animals. Emirates Journal of Food and Agriculture. 2023;35(3):203-9.
Kanwal R, Ahmed T, Mirza B. Comparative analysis of quality of milk collected from buffalo, cow, goat and sheep of Rawalpindi/Islamabad region in Pakistan. Asian Journal of Plant Sciences. 2004;3(3):300-5.
Chugh R, Kaur G. A Study on Milk Adulteration and methods of detection of various Chemical Adulterants qualitatively. InIOP Conference Series: Materials Science and Engineering 2022 (Vol. 1225, No. 1, p. 012046). IOP Publishing.
Memon MA, Khaskheli M, Kamboh AA, Soomro NA, Mangsi AS, Barham GS, Korejo NA. Surveillance of milk adulteration and its influence on physico-chemical characteristics of milk in Hyderabad, Pakistan. A Review. J. Anim. Health Prod. 2018;6(1):5-12.
Barham GS, Khaskheli M, Soomro AH, Nizamani ZA. Adulterated milk used for consumption in Thatta District of Sindh, Pakistan. American Journal of Agricultural Science. 2015;2(3):91-6.
Haasnoot W, Smits NG, Kemmers-Voncken AE, Bremer MG. Fast biosensor immunoassays for the detection of cows' milk in the milk of ewes and goats. Journal of Dairy Research. 2004;71(3):322-9.
Sadia A, Jabbar MA, Deng Y, Hussain EA, Riffat S, Naveed S, Arif M. A survey of aflatoxin M1 in milk and sweets of Punjab, Pakistan. Food control. 2012;26(2):235-40.
Jawaid S, Talpur FN, Nizamani SM, Afridi HI. Contamination profile of aflatoxin M1 residues in milk supply chain of Sindh, Pakistan. Toxicology reports. 2015;2:1418-22.
Yunus AW, Imtiaz N, Khan H, Ibrahim MN, Zafar Y. Aflatoxin contamination of milk marketed in Pakistan: A longitudinal study. Toxins. 2019;11(2):110.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Pak-Euro Journal of Medical and Life Sciences

This work is licensed under a Creative Commons Attribution 4.0 International License.




