In Vitro Evaluation of Antimicrobial Efficacy of Azadirachta Indica (Neem) extract against Staphylococcus aureus isolates from Subclinical Mastitis in Cholistani Cattle
DOI:
https://doi.org/10.31580/pjmls.v7isp2.3179Keywords:
Antibiotics, Azadirachta indica, Bacterial culture, disc diffusion method, Subclinical mastitis, Surf field mastitis test, S. aureus.Abstract
Subclinical mastitis (SCM) is a disease of mammary glands which usually affects dairy cattle during lactation. It causes huge economic impact to the dairy industry in terms of less milk yield and increased treatment cost. Mastitis pathogens mainly including Staphylococcus aureus is major pathogen frequently isolated from infected cow’s milk and possesses antimicrobial resistance against various antibiotics. Hence, current study was designed to detect SCM in lactating cows and to check in vitro antimicrobial efficacy of Azadirachta indica ((A. indica) against S. aureus isolates of mastitis milk. For this purpose, 358 milk samples were tested through surf field mastitis test (SFMT) and further confirmed through bacterial culture on mannitol salt agar. Positive milk samples 163/358 (45.53%) were detected through SFMT and 45/163 (27.6%) were later confirmed through bacterial culture. Several risk factors such as age, parity number, diameter of teat apex, total milk yield and folded thumb milking were significantly (P<0.05) associated with occurrence of SCM. Efficacy of different concentrations of methanolic extracts of A. indica i.e. 500mg/ml; 250mg/ml; 125mg/ml; 62.5mg/ml; 31.25mg/ml and frequently used antibiotics were checked against S. aureus isolates by disc diffusion method. The results revealed that S. aureus isolates were found sensitive to 500mg/ml concentration of A. indica extracts and to antibiotics including Ciprofloxacin, Septran and Tylosin. While isolates showed resistance against the Oxytetracycline, Oxacillin and Cefoxitin antibiotics. Hence, based on the results it can be concluded that the methanolic extracts of A. indica can be used as an alternative therapy in treating cow mastitis.
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