Telomere Length Variations in Correlations with Age and Physiological Destress in Hypertensive Patients in Quetta City, Balochistan
DOI:
https://doi.org/10.31580/d25jfd89Keywords:
Aging, Hypertension, qPCR, Telomere length, T/S Ratio, WizPrep gDNA Mini KitAbstract
The telomeric sequence, composed of repeated six-nucleotide units (TTAGGG), plays a crucial role in cellular ageing. Telomere length generally decreases with age but can also be influenced by factors such as gender, body mass index (BMI), medication, and lifestyle choices. In this study, DNA was extracted using the WizPrep™ gDNA Mini Kit (Blood) and amplified via quantitative polymerase chain reaction (qPCR) to determine cycle threshold values. These values were used to estimate the average telomere length for each DNA sample.
Among the hypertensive individuals analyzed (n=50), a negative correlation between age and telomere length was observed in the majority (68%). However, specific factors contributed to deviations from this pattern. Gender emerged as the most significant variable, with females exhibiting longer telomeres (average: 6,718 base pairs) compared to males (average: 5,914 base pairs), accounting for 16% of the observed deviations. BMI also influenced telomere length, as shorter telomeres were noted in obese individuals and longer telomeres in underweight individuals, affecting 12% of the samples. Lifestyle factors such as smoking (smokers: 5,816 base pairs; non-smokers: 6,596 base pairs) and physical activity (higher activity correlated with longer telomeres) were linked to telomere length variations, contributing to deviations in 2% of cases.
Despite these findings, the effects of other variables such as medication, diabetes, and dietary habits on telomere length remain unclear and warrant further research.
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