Antimicrobial Succeptibility Profile Of Escherichia Coli Among Urinary Tract Infections In Quetta City
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DOI:
https://doi.org/10.31580/mbk24n81Keywords:
Antimicrobial resistance, E. coli, Multi drug resistance, urinary tract infectionsAbstract
Drug resistance is becoming a cause of difficult situations around the globe. This resistance causes major difficulties in treatment. This entire study was carried out to detect the current level of Antibiotic Resistance in E. coli among urinary tract infected patients in Quetta. A total of 100 urine samples were collected randomly from different diagnostic laboratories of Quetta during May-June 2022. Organism detection was done by using specific media and various biochemical test including gram stain, catalase test, urease test, and methyl red test according to standard procedures. Antibiotic susceptibility test was done using some commonly used antibiotics including Amoxicillin-clavulanic acid, Ceftriaxone (CRO), Amikacin (AK), Ofloxacin (OFX), Imipenem (IPM) and Cefotaxime-Avibactam (CZA) on Mueller Hinton agar. Results indicated that E. coli isolates were more susceptible to Amikacin followed by Ceftazidime-avibactam, Imipenem, Ofloxacin and Ceftriaxone. While high resistance was observed against ceftriaxone followed by Ofloxacin, Ceftazidime-avibactam, Imipenem and Amikacin, respectively. This Most common UTI pathogen represent major Multidrug resistance against commonly prescribed antibiotics. Concluding the need of proper antimicrobial test before using antibiotics for treatment.References
Gupta K, Hooton T. M, Stamm W. E. Increasing Antimicrobial Resistance and the Management of Uncomplicated Community-Acquired Urinary Tract Infections. Annals of Internal Medicine.2001;135(1): 41-50.
Kang CI, K Jieum, WP dae, k Baek-Nam, Ha U-Syn, L seung-Ju, Y Jeong Kyun, M Seung Ki, L Heeyung, W Seong-Heon. Clinical Practice Guidelines for the Antibiotic Treatment of Community-Acquired Urinary Tract Infections. Infection and Chemotherapy. 2018;50(1): 67
Hooton T. M. Uncomplicated urinary tract infection. New England Journal of Medicine. 2012;366(11):1028-1037.
Nicolle, L. E. Urinary tract pathogens in complicated infection and in elderly individuals. The Journal of infectious diseases. 2001;183,S5-S8.
Sabir S, A.A, Anjum, T Ijaz, M.A Ali,MR Khan,M Nawaz. Isolation and antibiotic susceptibility of E. coli from urinary tract infections in a tertiary care hospital. Pakistan journal of medical sciences. 2014;30(2): 389.
Gajdacs M and Urban E. Resistance Trends and Epidemiology of Citrobacter-Enterobacter-Serratia in Urinary Tract Infections of Inpatients and Outpatients (RECESUTI): A 10-Year Survey. Medicina. 2019;55(6):285. https://doi.org/10.3390/medicina55060285
Gajdacs M, Abrok M, Lazar A, Burian K. Comparative epidemiology and resistance trends of common urinary pathogens in a tertiary-care hospital. a 10-year surveillance study. Medicina.2019;55(7):356.
Medina, E, pieper D.H. Tackling threats and future problems of multidrug-resistant bacteria. How to overcome the antibiotic crisis: facts, challenges, technologies and future perspectives. 2016;3-33. https://link.springer.com/chapter/10.1007/82_2016_492.
Kostakioti M, Hultgren S.J, Hadjifrangiskou M. Molecular blueprint of uropathogenic Escherichia coli virulence provides clues toward the development of anti-virulence therapeutics. Virulence. 2012;3(7):592-593.
Dash S. K, Chakraborty S.P, Mandal D, Roy S. Isolation and characterization of multi drug resistant uropathogenic Escherichia coli from urine sample of urinary tract infected patients. International Journal of Life Science and Pharma Research. 2012;2(1): 25-39.
Disassa N, Sibhat B, Mengistu S, Muktar Y, Belina D. Prevalence and antimicrobial susceptibility pattern of E. coli O157: H7 isolated from traditionally marketed raw cow milk in and around Asosa town, western Ethiopia. Veterinary medicine international, 2017. https://doi.org/10.1155/2017/7581531.
Akbar A, Naeem W, Liaqat, F, Sadiq M. B, Shafee M, Gul Z, Sher H. Hospital Acquired Pathogenic Escherichia coli from Clinical and Hospital Water Samples of Quetta Balochistan. Journal of Tropical Medicine. 2022; 1-8.
Okeke I. N, Fayinka S.T, Lamikanra A. Antibiotic resistance in Escherichia coli from Nigerian students, 1986 1998. Emerging Infectious Diseases.2000; 6(4): 393.
MacFaddin J. F. Biochemical tests for identification of medical bacteria williams and wilkins. Philadelphia. 2000; PA, 113(7).
Sefton, A. M. The impact of resistance on the management of urinary tract infections. International journal of antimicrobial agents.2000; 16 (4): 489-491.
Odongo, I, Ssemambo R, Joseph K. Prevalence of Escherichia coli and its antimicrobial susceptibility profiles among patients with UTI at Mulago Hospital, Kampala, Uganda. Interdisciplinary perspectives on infectious diseases, 2020.Chicago https://www.hindawi.com/journals/IPID/2020/8042540/
O'Neill, Jim. Tackling drug-resistant infections globally: final report and recommendations.2016 http://www.advancedinvestor.com/resources/ResearchMaterials/ Disease/ UKAMR_Report_Final.pdf
Mengal, H. M, Pirkani G.S, Ramzan S, Aziz S, Ahmad F, Nisar S, Azam S. Susceptibility Pattern of E. coli Isolates from Urinary Tract Infections, in Quetta: Susceptibility pattern of E. coli isolates from Urinary Tract Infections, in Quetta. Pak-Euro Journal of Medical and Life Sciences.2022;5(2):513-518.
Liao, Chun-Hsing L, Yao LN, Hung J.T, Susan S.J.L, Chin-Fu L, Po-Liang, Jiunn WU J, C.K.Wen C.K, Wen S.L,Po R.H. Antimicrobial activities of ceftazidime–avibactam, ceftolozane–tazobactam, and other agents against Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolated from intensive care units in Taiwan: results from the Surveillance of Multicenter Antimicrobial Resistance in Taiwan in 2016." Infection and Drug Resistance.2019;545-552.
Bashir M. F, Qazi J.I, Ahmad N, Riaz S. Diversity of urinary tract pathogens and drug resistant isolates of Escherichia coli in different age and gender groups of Pakistanis. Tropical Journal of pharmaceutical research, 2008; 7(3):1025-1031.
Winkler M. L, K.M, Wallace P, Bonomo R.A. Activity of ceftazidime/avibactam against isogenic strains of Escherichia coli containing KPC and SHV β-lactamases with single amino acid substitutions in the Ω-loop. Journal of Antimicrobial Chemotherapy. 2015; 70 (8): 2279-2286.
Dias Neto JA, Silva LD, Martins AC, Tiraboschi RB, Domingos AL,Suaid HJ, Tucci Jr S, Cologna AJ. Prevalence and bacterial susceptibility of hospital acquired urinary tract infection. Acta Cirurgica Brasileira.2003; (18): 36-38.
Ferdosi-Shahandashti E, Javanian M, Moradian-Kouchaksaraei M, Yeganeh B, Bijani A, Motevaseli E, Moradian-Kouchaksaraei F. Resistance patterns of Escherichia coli causing urinary tract infection. Caspian Journal of Internal Medicine.2015; 6 (3):148.
Rehmatullah, K, Ghulam R, Ghulam Mustafa S, Noman UH, Nisar AS, Muhammad A, Raseed A, Abdul G, Naila M and Abdullah. Quality control tests of Different levofloxacin and Ofloxacin Ofloxacin in the Market of Quetta city. International Journal of Current Research. 2021; 13(08): 18538-18543.
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