Isolation, Identification, Molecular Characterization and Antibiotic Susceptibility Testing of Uro- Pathogenic E.Coli (UPEC) Isolated From Non-Hospitalized Urinary tract infections (UTI)
DOI:
https://doi.org/10.31580/pjmls.v2i4.1140Keywords:
Urinary tract infection, UPEC, CLSI, tetracycline, norfloxacin, ampicillinAbstract
Background of the study: Urinary tract infections (UTIs) caused by Uro-pathogenic E.coli (UPEC) considered as most serious infections with increased mortality and morbidity. The ability of UPEC to encode variety of virulence determinants correlated with high recurrence rates and antibiotic resistance.
Purpose of the study: The present study focuses on DNA Extraction from E.coli by rapid PCR method and also characterization of Emboli’s molecules. E coli has the carrying ability of many mobile genes, these mobile genes carry the virulence factors. The present study was designed to detect these virulence genetic factors, using phenotypic method like multiplex PCR and detecting capsule synthesis, invasions toxin’s, Adhesions and side-rophores, The study also focused on a specific gene CHUA having ability in heme iron acquisition system and investigation of various virulence determinants expressed by UPEC and their relationship with antibiotic resistance.
Methods: Total 15 clinical samples of UPEC were isolated, identified and screened for antibiotic susceptibility pattern. Kirby Bauer disc diffusion test and micro broth dilution method were used to measure the antibiotic sensitivity testing of UPEC isolates. The susceptibility was tested by measuring the zone size after impregnated with antibiotic discs.
Results: The interpretation of zone size was done according to the proposed protocol of Clinical Laboratory and Standard Institute (CLSI). Majority of UPEC isolates (22%) were sensitive to tetracycline followed by Norfloxacin (18%). However, least sensitivity was observed against ampicillin (2%) and no sensitivity was experienced against cephalosporin (0%) and penicillin (0%).
Conclusion: It has been concluded that majority of UTI patient were suffering from UPEC. Resistance of UPEC against frontline drugs increasing rapidly. Thereby rational and appropriate use of antibiotics is the only way to save important therapeutic options.
