Optimization and Development of DNA-Based Biosensor for the Detection of Pathogens Research Article
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Abstract
The COVID-19 outbreak caused by SARS-CoV-2 disrupted global life and economies, but also spurred innovation in rapid diagnostics. DNA/RNA-based tools such as CRISPR and LAMP, along with AI-driven methods, have improved pathogen detection speed and accuracy. Among these, DNA biosensors are emerging as low-cost, efficient, and reliable diagnostic tools.
In this study, a DNA-based biosensor was developed and tested using carbon and gold electrodes to determine optimal biomolecule adherence, analyzed via TEM. Electrochemical impedance data were obtained using PCR-amplified positive and negative COVID-19 samples on a locally designed impedometer. Results indicated that gold electrodes exhibited superior signal performance compared to carbon electrodes. These findings provide a basis for further optimization and indigenization of DNA biosensors for rapid and cost-effective pathogen detection.
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