Phenotypic and Genotypic Insights: Isolation of Pseudomonas Aeruginosa from Burn Wounds Infections

Authors

  • Muhammad Farhan Mukhtar Department of Microbiology and Molecular Genetics, Bahauddin Zakariya University, Multan, Pakistan
  • Umar Zeb Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan
  • Muhammad Asad School of Biological Sciences, University of Padua, Padua, Italy
  • Kashif Ali Chaudhary School of Biological Sciences, University of Padua, Padua, Italy
  • Muhammad Akram Department of Chemical Pathology, Shaikh Zayd Hospital, Lahore, Pakistan

DOI:

https://doi.org/10.31580/pjmls.v7i4.3181

Keywords:

Aminoglycoside resistance, Burn wound infections, Genotypic analysis, Multidrug resistance, Pseudomonas aeruginosa

Abstract

Introduction: The Pseudomonas aeruginosa is gram negative pathogen commonly associated with burn wound infections. It poses a serious clinical challenge due to its resistance to aminoglycosides. 

Objective: Analyzing phenotypic and genotypic insights to P. Aeruginosa. Methodology: Wound swab samples were collected from 80 burn patients following ethical approval (ERC/01/04/2023). Microbiological techniques according to the Clinical and Laboratory Standards Institute (CLSI) were used to identify bacteria. The Miniprep bacterial DNA extraction kit was used to extract the DNA, and then PCR amplification was performed to target aminoglycoside resistance genes, such as aac(6')-Ib and aph(3')-IIa. The phenotype pattern of resistance was assessed by antibiotic susceptibility testing. Patient demographics data were analyzed, using SPSS, and with a focus on age, gender, and clinical severity.

Results: 50 P.aeruginosa isolates were Confirmed. Phenotypic analysis was performed and 80% of isolates exhibited multidrug resistance, particularly aminoglycoside resistant. The aac(6’)-Ib and aph(3’)-IIa genes were identified in 70 and 65 per cent of resistant isolates, respectively, through genotypic analysis. Taken together, these results suggest a positive correlation between phenotypic resistance and the presence of resistance genes.

Conclusion: The findings highlight the high prevalence of aminoglycoside resistance in P.aeruginosa isolated from burn wound infections, making it prototypical of resistance in clinical isolates. This points to the pressing need for alternate therapies and strict antimicrobial stewardship programs to mitigate the development of resistance.

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Published

2024-12-29