Screening Bacillus strains for Auxin Production and Their Potential to Stimulate the Growth of Vigna Radiata (L.)
DOI:
https://doi.org/10.31580/pjmls.v7i3.3132Keywords:
Bio fertilizer, Phosphate solubilizing microorganism, Plant Growth-promoting rhizobacteria, Vigna radiata (L.)Abstract
Auxin producing rhizobacteria are known to enhance plant growth and play a major role in the development of agronomical growth parameters. The main objective of this study was to evaluate the potential of Bacillus strains on the growth of Vigna Radiata (L.) under natural environmental conditions. The Microscopic analysis showed that strains were gram-positive and endospore former. Biochemical analysis revealed positive results for catalase and citrate test while negative for oxidase, urease, nitrate, TSI, and starch hydrolysis. The strains except Z-16 were tested negative for phosphate solubilization. Colourimetric analysis of strains at 0µg/ml of L-tryptophan recorded very low levels of auxin production. However, at 1000 µg/ml L-tryptophan, maximum auxin was produced by Bacillus subtilis Z-16 which was 85% over control. For pot trials surface sterilized seeds were treated with bacteria suspension and were sown into pots under natural environmental conditions. These trials resulted in maximum shoot length by strain of Bacillus subtilis Z-16 (85%), after 8 weeks of germination. Similarly, for fresh weight, B. subtilis produced recorded a 34% increase, over control. Analysis of dry weight proved that B. aerius Z-54 was giving maximum weight after 8 weeks of germination (12%). A mixture of Z-03 and Z-54 produced a 27% improvement in shoot length while a 1-fold improvement in fresh weight. The analysis of all these vegetative growth characteristics proved that these strains can be used as effective bio fertilizers to enhance crop productivity.
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