Phytochemical Screening and In-Vitro Evaluation of Antidiabetic Efficiency in Bioactive Extracts of Abelmoschus Esculentus and Momocardiac Charantia

Nimra Nawaz; Farah Naz Channa; Zainia Rehmat; Naheed  Sajjad; Seemab Zahra; Neha Mahreen Ali; Sadus; Maria Hassan

doi:10.31580/0tzfbp80

Authors

Nimra Nawaz Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan
Farah Naz Channa Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan https://orcid.org/0000-0003-3282-527X
Zainia Rehmat Department of Microbiology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan
Naheed Sajjad Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan https://orcid.org/0000-0001-6057-4637
Seemab Zahra Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan
Neha Mahreen Ali Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan
Sadus Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan
Maria Hassan Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan

DOI:

https://doi.org/10.31580/0tzfbp80

Keywords:

Alpha-amylase inhibition, Bioactive extracts, Bitter Gourd, Diabetes Mellitus, Okra, Phytochemicals Screening, Antidiabetic efficiency

Abstract

Diabetes mellitus (DM) is a severe, protracted, and complicated metabolic illness with various etiologies and life-altering short-term and long-term repercussions. People in both developed and developing countries are impacted by DM, which poses a serious socioeconomic problem. Pakistan has the third highest frequency of diabetes in the world after China and India. In response to the rising number of diabetic patients in Pakistan, the current study was designed to determine the in vitro antidiabetic efficiency of Abelmoschus esculentus and Momordica charantia, using inhibition of α-amylase and their phytochemical constituents. Extractions of these plants showed gradual increase in the α-amylase inhibition. The highest inhibition percentage of α-amylase obtained at highest concentration (5mg/ml) was 22% in A. esculentus and 26% in M. charantia. IC₅₀ value obtained was 10.1mg/ml in A. esculentus and 10.5mg/ml in M. charantia. M. charantia has a slightly better inhibition percentage of α-amylase than. A. esculentus. These two must function as an affordable medication for DM with fewer side effects. Most of the tests performed to test phytochemicals in both plants came positive, indicating that phytochemicals may also have some relationship to antidiabetic action because of their capacity to scavenge free radicals.

Author Biographies

Nimra Nawaz, Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan

Department of Biotechnology, Sardar Bahadur Khan Women University
Farah Naz Channa, Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan

Assistant Professor,

Biotechnology Department
Zainia Rehmat, Department of Microbiology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan

Assistant Professor,

Department of Microbiology, Sardar Bahadur Khan Women University
Naheed Sajjad, Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan

Associate Professor,

Department of Biotechnology,

Sardar Bahadur Khan Women University
Seemab Zahra, Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan

Department of Biotechnology, Sardar Bahadur Khan Women University
Neha Mahreen Ali, Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan

Department of Biotechnology,

Sardar Bahadur Khan Women University
Sadus, Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan

Department of Biotechnology,

Sardar Bahadur Khan Women University
Maria Hassan, Department of Biotechnology, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan

Department of Biotechnology

Sardar Bahadur Khan Women University

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