Quantification of Carbohydrates, Proteins and Antioxidant Enzymes in Withania Coagulans from Balochistan

Misbah Javed; Ayesha Mushtaq; Farida Behlil; Farrukh Bashir; Musarrat Riaz; Shagufta Fehmid; Fazeela Mandokhail; Abdul Haq

doi:10.31580/pjmls.v7i2.2944

Authors

Misbah Javed Department of Chemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan
Ayesha Mushtaq Department of Bio-Chemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan https://orcid.org/0000-0002-2010-2130
Farida Behlil Department of Chemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan
Farrukh Bashir Department of Chemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan
Musarrat Riaz Department of Chemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan
Shagufta Fehmid Department of Chemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan
Fazeela Mandokhail Department of Chemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan
Abdul Haq Directorate of Water Management (HEIS), Agriculture Research Institute (ARI), Sariab Road, Quetta, Pakistan

DOI:

https://doi.org/10.31580/pjmls.v7i2.2944

Keywords:

Catalase, Extraction, Peroxidase, Superoxide dismutase, Withania coagulans

Abstract

Withania coagulans is getting very popular due to its medicinal properties. The present work was intended to quantify the level of carbohydrates, proteins and antioxidant enzymes including Superoxide dismutase, Catalase and Peroxidase in the herbal medicinal plant Withania coagulans collected from different areas of Balochistan. To check the presence of carbohydrates, seeds of Withania coagulans were treated with Fehling Reagent which confirmed the presence of reducing sugars. The carbohydrate quantification was done using the Anthrone method and finally, absorbance was checked at 630 nm. Quantification of proteins was conducted by exercising Bradford Method and absorbance was measured at 595 nm by using a spectrophotometer. Enzymatic extraction from seeds and leaves was accomplished by using phosphate buffer and centrifugation at 1500 rpm for twenty minutes. Superoxide dismutase level was assessed by utilizing the Giannopolitis & Ries method whereas catalase and peroxidase activities were determined by employing the strategy of Chance & Maehly with certain modifications. The results disclosed a mean carbohydrate level of 6.27 mg/kg which signifies its hypoglycemic properties. The mean protein contents for seeds and leaves are 265.67 mg/kg and 186.33 mg/kg respectively. Superoxide dismutase contents are found to be the highest among all three antioxidant enzymes. Hence, it can be concluded that Withania coagulans due to the presence of different phytochemical constituents has several protective and medicinal uses.

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