Isolation, Identification, and Molecular Characterization of Streptococcus Species from Female Acne Patients in Quetta City
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Abstract
The skin, body’s largest organ, which contains millions of microorganisms, is susceptible to various conditions, including acne vulgaris. While most skin microorganisms are beneficial, certain strains may contribute to dermatological issues. Acne, particularly prevalent among teenagers, involves inflammatory responses in the sebaceous glands. This study focused on unmarried female acne patients in Quetta City, aiming to identify bacterial pathogens associated with acne lesions. Pus samples from 100 patients were collected at BMC Quetta’s dermatology OPDs. Both PCR and conventional methods were employed for pathogen identification. Results indicated P. acne isolation in 56% of patients through conventional methods and in 65% of patients through PCR methods. S. epidermidis was identified in 71% and 79% of the samples using conventional and PCR methods, while S. aureus detection rates were 76% (conventional method) and 83% (PCR). Furthermore, S. pyogenes was detected in 47% of patients through PCR and 32% of patients using conventional methods. These findings underscore the significance of specific streptococcus species in acne pathogenesis. Such insights contribute to enhance dermatological understanding and targeted treatment approaches.
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