Isolation, Identification and In-Vitro Management of Soft Rot of Onion in Balochistan
DOI:
https://doi.org/10.31580/3a31jr76Keywords:
Management, Onion, Pathogenicity, Phenotypic diversity, Soft rootAbstract
The onion, a crucial vegetable crop widely utilized in households, is susceptible to various biotic and abiotic factors throughout cultivation and storage. The escalating prevalence of onion soft rot presents a substantial economic threat on a global scale, leading to considerable losses during post-harvest and storage phases, particularly in Asian regions. Therefore, early identification and effective management of this deteriorating disease are imperative. Through comprehensive biochemical and physiological assessments, including Gram staining, catalase testing, starch hydrolysis, NaCl tolerance (5.0%), pH tolerance (8.0), yeast dextrose chloramphenicol agar (YDC), growth at 37˚C, and potassium hydroxide (KOH, 3%), the causative agent of onion soft rot was identified as Dickeya dadantii spp. In the pursuit of di sease management, seven antibiotics were administered at concentrations of 100 ppm, 200 ppm, and 300 ppm. Tetracycline exhibited the highest efficacy, manifesting a notable 34.08% inhibition zone. Conversely, Penicillin-streptomycin displayed diminished effectiveness, with a minimal inhibition zone of 1.16%. Despite the in-vitro nature of the study, the compelling results advocate for the application of Tetracycline as a preferred option for the agricultural community in mitigating onion soft rot, emphasizing its minimal risk to human health.
References
Necola S. Allium cepa variety evaluation for organic farming in the 5th int. symposium on Edible Allianceae. Dronten the Nether lands. ISHS. 2007 45.
Khokhar KM. Environmental and genotypic effects on bulb development in onion–a review. The Journal of Horticultural Science and Biotechnology. 2017;92(5):448-54.
Griffiths G, Trueman L, Crowther T, Thomas B, Smith B. Onions: a global benefit to health. Phytother Res. 2002;16(7):603–615.
Ponvizhi Ramya V, Gunasekaran S, Senthil Kumar M. Genetic Diversity of Soft Rot Pathogens in Potato of South Indian Origin. Research Journal of Biotechnology Vol. 2014;9:5.
Schwartz HF, Mohan SK. Compendium of onion and garlic diseases and pests. Compendium of onion and garlic diseases and pests. 2007(Ed. 2).
Wright PJ, Clark RG, Hale CN. A storage soft rot of New Zealand onions caused by Pseudomonas gladioli pv. alliicola. New Zealand journal of crop and horticultural science. 1993;21(3):225-7.
Agrios GN. Plant pathology 5th edition: Elsevier academic press. Burlington, Ma. USA. 2005:79-103.
Kumar V, Neeraj SS, Sagar NA. Post harvest management of fungal diseases in onion—a review. International Journal of Current Microbiology and Applied Sciences. 2015;4(6):737-52.
Kim YK, Lee SD, Park CS, Lee SB, Lee SY. Soft rot of onion bulbs caused by Pseudomonas marginalis under low temperature storage. The Plant Pathology Journal. 2002;18(4):199-203.
Gupta SK, Thind TS. Disease problems in vegetable production. Scientific Publishers; 2018 Apr 1.
Liao CH, Wells JM. Diversity of pectolytic, fluorescent pseudomonads causing soft rots of fresh vegetables at produce markets. Phytopathology. 1987;77(5):673-7.
Samson R, Legendre JB, Christen R, Saux MF, Achouak W, Gardan L. Transfer of Pectobacterium chrysanthemi (Burkholder et al. 1953) Brenner et al. 1973 and Brenneria paradisiaca to the genus Dickeya gen. nov. as Dickeya chrysanthemi comb. nov. and Dickeya paradisiaca comb. nov. and delineation of four novel species, Dickeya dadantii sp. nov., Dickeya dianthicola sp. nov., Dickeya dieffenbachiae sp. nov. and Dickeya zeae sp. nov. International Journal of Systematic and Evolutionary Microbiology. 2005;55(4):1415-27.
Costechareyre D, Balmand S, Condemine G, Rahbé Y. Dickeya dadantii, a plant pathogenic bacterium producing Cyt-like entomotoxins, causes septicemia in the pea aphid Acyrthosiphon pisum. PLoS One. 2012;24;7(1):e30702.
Cother EJ, Gilbert RL. Presence of Erwinia chrysanthemi in two major river systems and their alpine sources in Australia. Journal of Applied Microbiology. 1990; 1;69(5):729-38.
Grenier AM, Duport G, Pages S, Condemine G, Rahbé Y. The phytopathogen Dickeya dadantii (Erwinia chrysanthemi 3937) is a pathogen of the pea aphid. Applied and Environmental Microbiology. 2006;72(3):1956-65.
Sławiak M, Łojkowska E, Van der Wolf JM. First report of bacterial soft rot on potato caused by Dickeya sp.(syn. Erwinia chrysanthemi) in Poland. Plant Pathology. 2009;58(4).
Abdalla ME, Hamza S, Fayzalla SA, Seeda SE. Post harvest control of bacterial soft rot pathogens of onion bulb in storage. Journal of Plant Protection and Pathology. 2013;4(11):945-57.
Bartz JA, Kelman A. Reducing the potential for bacterial soft rot in potato tubers by chemical treatments and drying. American potato journal. 1986;63:481-93.
Bonde R, de Souza P. Studies on the control of potato bacterial seed-piece decay and blackleg with antibiotics. American Potato Journal. 1954;31(10):311-6.
Harris, R. I. Chemical control of bacterial soft-rot of wounded potato tubers. Potato Research. 1979;22, 245-249.
Shing R S. Diseases of Vegetable crops. Oxford and IBA Publishing Company Pvt. Ltd. New Delhi. 1985;331pp.
Coventry MJ, Gordon JB, Wilcock A, Harmark K, Davidson BE, Hickey MW, Hillier AJ, Wan J. Detection of bacteriocins of lactic acid bacteria isolated from foods and comparison with pediocin and nisin. Journal of Applied Microbiology. 1997; 83(2):248-58.
Suslow TV, Schroth MN, Isaka M. Application of a rapid method for gram differentiation of plant pathogenic and saprophytic bacteria without staining. Phytopathology. 1982;72(7):917-8.
Reiner K. Catalase test protocol. American society for microbiology. 2010;11:1-6.
Cowan ST, Steel KJ. Manual for the identification of medical bacteria. (No Title). 1974.
Patel A, Prajapati JB, Holst O, Ljungh A. Determining probiotic potential of exopolysaccharide producing lactic acid bacteria isolated from vegetables and traditional Indian fermented food products. Food Bioscience. 2014; 1(5):27-33.
AL-Saleh MA. Pathogenic variability among five bacterial isolates of Xanthomonas campestris pv. vesicatoria, causing spot disease on tomato and their response to salicylic acid. Journal of the Saudi Society of Agricultural Sciences. 2011;10(1):47-51.
Pina-Pérez MC, González A, Moreno Y, Ferrús MA. Helicobacter pylori growth pattern in reference media and extracts from selected minimally processed vegetables. Food Control. 2018 Apr 1;86:389-96.
Schaad NW, Jones JB, Chun W. Laboratory guide for the identification of plant pathogenic bacteria. American Phytopathological society (APS press). 2001.
Naqqash T, Hameed S, Imran A, Hanif MK, Majeed A, van Elsas JD. Differential response of potato toward inoculation with taxonomically diverse plant growth promoting rhizobacteria. Frontiers in plant science. 2016;7:144.
Thornberry HH. A paper-disk plate method for the quantitative evaluation of fungicides and bactericides. Phytopathology. 1950;40(5).
Anonymous. Antibiotics. In: Drug Today. P.O. Box No. 9116 Delhi 110091, 2003; 218-232.
Bhattacharya I, Mukherjee N. Soft rot of storage tissue due to some uncommon bacteria: some epidemiological aspects. Indian Agriculturist. 1986;30(1); 75-82.
Raju MR, Pal V, Jalali I. Inoculation method of Pectobacterium carotovorum subsp. carotovorum and factors influencing development of bacterial soft rot in radish. J. Mycol. Plant Pathol. 2008;38:311-5.
Walker JC. Bacterial soft rots of carrot. Diseases of Vegetable Crops. Discovery Publishing Company, New Delhi. 1998;184-215.
Mills AA, Hurta RA. Sensitivity of Erwinia spp. to salt compounds in vitro and their effect on the development of soft rot in potato tubers in storage. Postharvest Biology and Technology. 2006; 41(2):208-14.
HIGASHIO H, Yamada M. Control of soft rot after harvest of cabbage in Indonesia. Japan Agricultural Research Quarterly: JARQ. 2004;38(3):175-8.
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