Metabolic Profiling of Cancer Cells Identifying Metabolic Vulnerabilities for Targeted Therapy in Patients of Quetta, Balochistan, Pakistan

Abstract

The current research was performed from March 2022 until June 2022 by following the guidelines of a joint clinical research committee of Bolan Medical Complex and the University of Balochistan Quetta. The ability to uncover new targets for cancer therapy has made metabolic profiling of cancer cells a crucial field of research in cancer biology. For many years, it has been common practice to utilize NMR spectroscopy, especially in vivo magnetic resonance spectroscopy (MRS) and high-resolution solution-state analysis of tissue extracts, to differentiate between various cell lines and tumor types. Metabolic profiling is the process of analyzing the unique metabolic characteristics of cancer cells compared to normal cells. The cells should be collected under sterile conditions and kept in a suitable medium until analysis. Cancer cells tend to consume large amounts of glucose, even in the presence of oxygen, leading to increased levels of lactate production. Cancer cells may exhibit changes in the regulation of lipid synthesis, storage, and utilization, which can impact cellular signalling and membrane function. By analyzing the metabolites in cancer cells and comparing them to normal cells, researchers can gain insights into the metabolic alterations that occur in cancer. Such cancer cells can be employed for many things, like diagnosis, prognosis, and the creation of fresh therapeutic approaches. In conclusion, metabolic profiling of cancer cells is a rapidly evolving field with the potential to improve our understanding of cancer biology and to identify new therapeutic strategies for cancer. It can be used to identify new targets for immunotherapy. Cancer cell's metabolic properties can affect how they interact with the immune system, and targeting metabolic pathways could enhance the efficacy of immunotherapies.