Detection and Characterization of Ornithobacterium Rhinotracheale in Commercial Layer Poultry of Metropolitan Lahore, Pakistan

Authors

  • Aroosha Hussain School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
  • Zahoor Qadir Samra School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
  • Amina Hussain School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan

DOI:

https://doi.org/10.31580/pjmls.v6i3.2857

Keywords:

Ornithobacterium rhinotracheale, respiratory disorders, veterinary diseases, Commercial poultry, avian disease

Abstract

Background: Ornithobacterium rhinotracheale (ORT) is a bacteria linked to respiratory illness, growth retardation, reduced egg output, and death in chickens. The poultry sector in Pakistan is helping to meet the protein needs. Respiratory illnesses are major issues in the poultry industry. A disease-free broiler breed is deemed beneficial to the food supply and economy. The aim of the present study was to detect and characterize the presence of ORT infection in commercial layer poultry of metropolitan Lahore using biochemical and molecular methods. Methods: This cross-sectional study collected 600 tracheal swab samples from commercial chicken shops in three groups (symptomatic, deceased, and physically healthy flocks; 200/ category) in Lahore, Pakistan. Results: The findings of the MacConkey agar test, the triple iron sugar test, and the oxidase test (confirmed by 16SrRNA PCR-analysis) offer a more accurate image of infection diagnosis compared to other biochemical approaches. The ORT infection was shown to be present at a rate of 39% (n = 78) in symptomatic flock samples, 36% (n = 72) in deceased flock samples, and 10.5% (n = 21) in physically healthy flock samples. Additionally, the resistance patterns of ORT isolates to a panel of routinely used antimicrobial drugs were assessed. Isolates were susceptible to tetracycline, florfenicol, and resistant to ciprofloxacin, ampicillin, amoxicillin, and gentamicin. Conclusion: The study concluded that the frequency of ORT infection (n= 600) in commercial poultry was 28.5 percent (n=171). This is the first report of its kind to identify and characterise ORT infection in commercial layer chicken in Lahore, Pakistan.

Author Biographies

  • Aroosha Hussain, School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
    Background: Ornithobacterium rhinotracheale (ORT) is a bacteria linked to respiratory illness, growth retardation, reduced egg output, and death in chickens. The poultry sector in Pakistan is helping to meet the protein needs. Respiratory illnesses are major issues in the poultry industry. A disease-free broiler breed is deemed beneficial to the food supply and economy. The aim of the present study was to detect and characterize the presence of ORT infection in commercial layer poultry of metropolitan Lahore using biochemical and molecular methods. Methods: This cross-sectional study collected 600 tracheal swab samples from commercial chicken shops in three groups (symptomatic, deceased, and physically healthy flocks; 200/ category) in Lahore, Pakistan. Results: The findings of the MacConkey agar test, the triple iron sugar test, and the oxidase test (confirmed by 16SrRNA PCR-analysis) offer a more accurate image of infection diagnosis compared to other biochemical approaches. The ORT infection was shown to be present at a rate of 39% (n = 78) in symptomatic flock samples, 36% (n = 72) in deceased flock samples, and 10.5% (n = 21) in physically healthy flock samples. Additionally, the resistance patterns of ORT isolates to a panel of routinely used antimicrobial drugs were assessed. Isolates were susceptible to tetracycline, florfenicol, and resistant to ciprofloxacin, ampicillin, amoxicillin, and gentamicin. Conclusion: The study concluded that the frequency of ORT infection (n= 600) in commercial poultry was 28.5 percent (n=171). This is the first report of its kind to identify and characterise ORT infection in commercial layer chicken in Lahore, Pakistan.
  • Zahoor Qadir Samra, School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
    Background: Ornithobacterium rhinotracheale (ORT) is a bacteria linked to respiratory illness, growth retardation, reduced egg output, and death in chickens. The poultry sector in Pakistan is helping to meet the protein needs. Respiratory illnesses are major issues in the poultry industry. A disease-free broiler breed is deemed beneficial to the food supply and economy. The aim of the present study was to detect and characterize the presence of ORT infection in commercial layer poultry of metropolitan Lahore using biochemical and molecular methods. Methods: This cross-sectional study collected 600 tracheal swab samples from commercial chicken shops in three groups (symptomatic, deceased, and physically healthy flocks; 200/ category) in Lahore, Pakistan. Results: The findings of the MacConkey agar test, the triple iron sugar test, and the oxidase test (confirmed by 16SrRNA PCR-analysis) offer a more accurate image of infection diagnosis compared to other biochemical approaches. The ORT infection was shown to be present at a rate of 39% (n = 78) in symptomatic flock samples, 36% (n = 72) in deceased flock samples, and 10.5% (n = 21) in physically healthy flock samples. Additionally, the resistance patterns of ORT isolates to a panel of routinely used antimicrobial drugs were assessed. Isolates were susceptible to tetracycline, florfenicol, and resistant to ciprofloxacin, ampicillin, amoxicillin, and gentamicin. Conclusion: The study concluded that the frequency of ORT infection (n= 600) in commercial poultry was 28.5 percent (n=171). This is the first report of its kind to identify and characterise ORT infection in commercial layer chicken in Lahore, Pakistan.
  • Amina Hussain, School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
    Background: Ornithobacterium rhinotracheale (ORT) is a bacteria linked to respiratory illness, growth retardation, reduced egg output, and death in chickens. The poultry sector in Pakistan is helping to meet the protein needs. Respiratory illnesses are major issues in the poultry industry. A disease-free broiler breed is deemed beneficial to the food supply and economy. The aim of the present study was to detect and characterize the presence of ORT infection in commercial layer poultry of metropolitan Lahore using biochemical and molecular methods. Methods: This cross-sectional study collected 600 tracheal swab samples from commercial chicken shops in three groups (symptomatic, deceased, and physically healthy flocks; 200/ category) in Lahore, Pakistan. Results: The findings of the MacConkey agar test, the triple iron sugar test, and the oxidase test (confirmed by 16SrRNA PCR-analysis) offer a more accurate image of infection diagnosis compared to other biochemical approaches. The ORT infection was shown to be present at a rate of 39% (n = 78) in symptomatic flock samples, 36% (n = 72) in deceased flock samples, and 10.5% (n = 21) in physically healthy flock samples. Additionally, the resistance patterns of ORT isolates to a panel of routinely used antimicrobial drugs were assessed. Isolates were susceptible to tetracycline, florfenicol, and resistant to ciprofloxacin, ampicillin, amoxicillin, and gentamicin. Conclusion: The study concluded that the frequency of ORT infection (n= 600) in commercial poultry was 28.5 percent (n=171). This is the first report of its kind to identify and characterise ORT infection in commercial layer chicken in Lahore, Pakistan.

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Published

2023-09-30

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Vol. 6 No. 3 (2023): July-September

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