Evaluation of Insecticide-Treated Nets Against Cx. Quinquefasciatus Mosquitoes in Punjab, Pakistan


  • Syed Shakeel Shah Department of Zoology, University of Narowal, Narowal, Pakistan; Institute of Zoology, University of Punjab, Lahore, Pakistan
  • Abida Butt Institute of Zoology, University of Punjab, Lahore, Pakistan
  • Sabila Afzal Department of Zoology, University of Narowal, Narowal, Pakistan; Institute of Zoology, University of Punjab, Lahore, Pakistan
  • Aneeqa Majeed Department of Zoology, University of Narowal, Narowal, Pakistan
  • Afifa Amjad Department of Zoology, University of Narowal, Narowal, Pakistan
  • Amna Aslam Butt Department of Zoology, University of Narowal, Narowal, Pakistan




Insecticide-treated Nets (ITNS), Insecticide Resistance, Mosquito-Borne Diseases, Mosquito Control, Sustainability


Background and objectives: Mosquitoes are well-known vectors of numerous diseases on a global scale. Culex quinquefasciatus, a widely recognized urban mosquito species, serves as a carrier for filarial parasites and various arboviruses. In the current study, the effectiveness of DawaplusR 2.0 ITNs against Cx. quinquefasciatus was assessed.

Methods: The larvae were collected from various habitats in the districts of Narowal and Gujrat, Punjab, Pakistan. The larvae were reared in the laboratory under the standard rearing conditions. For the purpose of assessing mosquito resistance towards ITNs, adult female mosquitoes were used following the WHO tunnel bioassay. The calculation was done to determine the percentages of mortality, passage, and blood-feeding inhibition.

Results: In comparison to the control group, the percentage of mortality, passage, and blood-feeding inhibition shows that the ITNs can provide protection against mosquitoes. However, their effectiveness in offering protection against resistant populations was limited.

Interpretation & conclusion: The findings of this study suggest that the ITNs employed exhibited a general reduction in the mosquito population. Depending on the site of collection, the effectiveness of nets varies. This study highlights the importance of considering contextual variables when implementing mosquito control interventions. More research and surveillance are needed to develop effective approaches for managing Cx. quinquefasciatus in different areas.


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