Evaluation of Insecticide-Treated Nets Against Cx. Quinquefasciatus Mosquitoes in Punjab, Pakistan
DOI:
https://doi.org/10.31580/x4f95x52Keywords:
Insecticide-treated Nets (ITNS), Insecticide Resistance, Mosquito-Borne Diseases, Mosquito Control, SustainabilityAbstract
Background and objectives: Mosquitoes are well-known vectors of numerous diseases on a global scale. Culex quinquefasciatus, a widely recognized urban mosquito species, serves as a carrier for filarial parasites and various arboviruses. In the current study, the effectiveness of DawaplusR 2.0 ITNs against Cx. quinquefasciatus was assessed.
Methods: The larvae were collected from various habitats in the districts of Narowal and Gujrat, Punjab, Pakistan. The larvae were reared in the laboratory under the standard rearing conditions. For the purpose of assessing mosquito resistance towards ITNs, adult female mosquitoes were used following the WHO tunnel bioassay. The calculation was done to determine the percentages of mortality, passage, and blood-feeding inhibition.
Results: In comparison to the control group, the percentage of mortality, passage, and blood-feeding inhibition shows that the ITNs can provide protection against mosquitoes. However, their effectiveness in offering protection against resistant populations was limited.
Interpretation & conclusion: The findings of this study suggest that the ITNs employed exhibited a general reduction in the mosquito population. Depending on the site of collection, the effectiveness of nets varies. This study highlights the importance of considering contextual variables when implementing mosquito control interventions. More research and surveillance are needed to develop effective approaches for managing Cx. quinquefasciatus in different areas.
References
Sargent K, Mollard J, Henley SF, Bollasina MA. Predicting Transmission Suitability of Mosquito-Borne Diseases under Climate Change to Underpin Decision Making. International journal of environmental research and public health. 2022;19 (20):13656.
Huang X, Kaufman PE, Athrey GN, Fredregill C, Alvarez C, Shetty V, Slotman MA. Potential key genes involved in metabolic resistance to malathion in the southern house mosquito, Culex quinquefasciatus, and functional validation of CYP325BC1 and CYP9M12 as candidate genes using RNA interference. BMC genomics. 2023;24(1):1-5.
Bhattacharya S, Basu P, Sajal Bhattacharya C. The southern house mosquito, Culex quinquefasciatus: profile of a smart vector. 2016;4(2):73-81.
WHO. Vector-Borne Diseases World Health Organization: World Health Organization. 2021.
Huynh LN, Tran LB, Nguyen HS, Ho VH, Parola P, Nguyen XQ. Mosquitoes and Mosquito-Borne Diseases in Vietnam. Insects. 2022;13(12):1076.
Srivastav AK, Kumar A, Srivastava PK, Ghosh M. Modeling, and optimal control of dengue disease with screening and information. The European Physical Journal Plus. 2021;136(11):1187.
Guy A. Studies on the impact of insecticide-treated nets on blood feeding and host seeking behavior of pyrethroid resistant Anopheles gambiae. The University of Liverpool (United Kingdom); 2021:1-160.
Skovmand O, Dang DM, Tran TQ, Bossellman R, Moore SJ. From the factory to the field: considerations of product characteristics for insecticide-treated net (ITN) bio efficacy testing. Malaria Journal. 2021;20:1-13.
Organization WHO. The evaluation process for vector control products. World Health Organization, 2017.
Nash RK, Lambert B, NʼGuessan R, Ngufor C, Rowland M, Oxborough R, Moore S, Tungu P, Sherrard-Smith E, Churcher TS. Systematic review of the entomological impact of insecticide-treated nets evaluated using experimental hut trials in Africa. Current research in parasitology & vector-borne diseases. 2021;1:1-13.
Akuffo R, Wilson M, Sarfo B, Dako-Gyeke P, Adanu R, Anto F. Insecticide-treated net (ITN) use, factors associated with non-use of ITNs, and occurrence of sand flies in three communities with reported cases of cutaneous leishmaniasis in Ghana. PloS one. 2021;16(12).
Rogerson SJ, Beeson JG, Laman M, Poespoprodjo JR, William T, Simpson JA, Price RN. Identifying and combating the impacts of COVID-19 on malaria. BMC medicine. 2020;18(1):1-7.
WHO. World malaria report 2020: 20 years of global progress and challenges. Geneva: World Health Organization. 2020.
Monroe A, Moore S, Olapeju B, Merritt AP, Okumu F. Unlocking the human factor to increase effectiveness and sustainability of malaria vector control. Malaria Journal. 2021;20(1):1-6.
Statistics PBO. Narowal District Pakistan Bureau of Statistics GOP; 2017.
GOP. Climate and Agriculture (Flora and Fauna): District Gujrat, Government of Punjab; 2023.
Arifa Z, Zaheer-ud-Din K, Almas J. Determination of correlation between plant distribution and ecological factors in Narowal district Punjab, Pakistan. Bangladesh Journal of Botany. 2018;47(3):451-8.
Rehman A, Hussain K, Nawaz K, Arshad N, Iqbal I, Ali SS, Nazeer A, Bashir Z, Jafar S, Arif U. Indigenous knowledge and medicinal significance of seasonal weeds of district Gujrat, Punjab, Pakistan. Ethnobotany Research and Applications. 2020;20:1-9.
Ye Z, Liu F, Sun H, Barker M, Pitts RJ, Zwiebel LJ. Heterogeneous expression of the ammonium transporter AgAmt in chemosensory appendages of the malaria vector, Anopheles gambiae. Insect biochemistry and molecular biology. 2020;120:103360.
Githinji EK, Irungu LW, Ndegwa PN, Machani MG, Amito RO, Kemei BJ, Murima PN, Ombui GM, Wanjoya AK, Mbogo CM, Mathenge EM. Impact of insecticide resistance on P. falciparum vectors’ biting, feeding, and resting behavior in selected clusters in Teso North and South Sub countries in Busia County, western Kenya. Journal of parasitology research. 2020; 2020: 1-11.
Harbach RE. The mosquitoes of the subgenus Culex in southwestern Asia and Egypt (Diptera: Culicidae). Contributions of the American Entomological Institute. 1988;24(1).
Reuben R, Tewari S, Hiriyan J, Akiyama J. Illustrated keys to species of Culex (Culex) associated with Japanese encephalitis in Southeast Asia (Diptera: Culicidae). Mosquito systematics. 1994;26(2):75-96.
WHO. Guidelines for laboratory and field testing of long-lasting insecticidal mosquito nets. World Health Organization 2005:1-15.
Kamande DS, Odufuwa OG, Mbuba E, Hofer L, Moore SJ. Modified World Health Organization (WHO) Tunnel test for higher throughput evaluation of insecticide-treated nets (ITNs) considering the effect of alternative hosts, exposure time, and mosquito density. Insects. 2022;13(7):562.
Machani MG, Ochomo E, Amimo F, Mukabana WR, Githeko AK, Yan G, Afrane YA. Behavioral responses of pyrethroid resistant and susceptible Anopheles gambiae mosquitoes to insecticide treated bed net. PloS one. 2022;17(4).
Oke PO, Terhemba KU, Msugh-Ter Manyi M, Ogbaje CI. Comparative study on the susceptibility status of three common mosquitoes species in Makurdi to eight different insecticides using who test tube bioassays. Animal Research International. 2022;19(2):4507–14.
Tahir HM, Butt A, Khan SY. Response of Culex quinquefasciatus to deltamethrin in Lahore district. Journal of Parasitology and Vector Biology. 2009;1(3):19-24.
Rafinejad J, Vatandoost H, Nikpoor F, Abai MR, Shaeghi M, Duchen S, Rafi F. Effect of washing on the bio-efficacy of insecticide-treated nets (ITNs) and long-lasting insecticidal nets (LLINs) against main malaria vector Anopheles stephensi by three bioassay methods. Journal of vector borne diseases. 2008;45(2):143.
Oxborough RM, Kitau J, Matowo J, Feston E, Mndeme R, Mosha FW, Rowland MW. ITN mixtures of chlorfenapyr (pyrrole) and alphacypermethrin (pyrethroid) for control of pyrethroid resistant Anopheles arabiensis and Culex quinquefasciatus. PLoS One. 2013;8(2).
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