Helicobacter Pylori Prevalence and Associated Risk Factors in Individuals with Recurrent Epigastric Pain: Lahore, Pakistan

Amina Hussain; Zahoor Qadir Samra; Aroosha Hussain

doi:10.31580/pjmls.v6i3.2849

Authors

Amina Hussain School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
Zahoor Qadir Samra School of Biochemistry and Biotechnology, The University of Punjab
Aroosha Hussain School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan

DOI:

https://doi.org/10.31580/pjmls.v6i3.2849

Keywords:

Epigastric pain, Frequency, Gastritis, Helicobacter pylori, Risk factors

Abstract

A number of stomach diseases, including gastritis, peptic ulcers, and gastric cancer, have been related to Helicobacter pylori (H. pylori), creating serious public health concerns. The current investigation aimed to determine the frequency of H. pylori infection and related risk factors in individuals having symptoms of recurring epigastric pain.

Methods: The present study involves 191 participants having recurring epigastric pain. Sociodemographic data, blood, and tissue samples were obtained from 112 patients between September 2020 to August 2021. Biochemical and molecular methods were used to estimate the infection frequency.

Results: The findings revealed that the overall infection ratio was 58% (n=65 out of 112). Males (n=41, 63.1%) contracted the infection more frequently than females. The participants belong to the 26-30 age group (n=37, 56.9%) low socioeconomic status (n=45, 69.2%), resided in rural areas (n=54, 83.1%), and consumed unfiltered water (n=57, 87.7%) had observed with significantly higher infection rates. The regression analysis demonstrated greater probability of contracting infection in male gender (OR 5.91, 95%CI: 2.40-12.9), in 26-35 age group (OR 6.16, 95%CI: 1.75-21.6), tap water users (OR 4.42, 95%CI: 1.72-11.3), rural area residents (OR 3.31, 95%CI:1.39-9.76), and low economic status group (OR 5.26, 95%CI:1.84-16.5) as p< 0.05).

Conclusion: The findings revealed the incidence of H. pylori infection (58%) and its associated risk factors in individuals having recurring epigastric pain. The results suggest that larger datasets are needed for future studies to accurately reflect infection frequency and risk factors to diagnose and treat the disease.

Author Biographies

Amina Hussain, School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan

A number of stomach diseases, including gastritis, peptic ulcers, and gastric cancer, have been related to Helicobacter pylori (H. pylori), creating serious public health concerns. The current investigation aimed to determine the frequency of H. pylori infection and related risk factors in individuals having symptoms of recurring epigastric pain.Methods: The present study involves 191 participants having recurring epigastric pain. Sociodemographic data, blood, and tissue samples were obtained from 112 patients between September 2020 to August 2021. Biochemical and molecular methods were used to estimate the infection frequency.Results: The findings revealed that the overall infection ratio was 58% (n=65 out of 112). Males (n=41, 63.1%) contracted the infection more frequently than females. The participants belong to the 26-30 age group (n=37, 56.9%) low socioeconomic status (n=45, 69.2%), resided in rural areas (n=54, 83.1%), and consumed unfiltered water (n=57, 87.7%) had observed with significantly higher infection rates. The regression analysis demonstrated greater probability of contracting infection in male gender (OR 5.91, 95%CI: 2.40-12.9), in 26-35 age group (OR 6.16, 95%CI: 1.75-21.6), tap water users (OR 4.42, 95%CI: 1.72-11.3), rural area residents (OR 3.31, 95%CI:1.39-9.76), and low economic status group (OR 5.26, 95%CI:1.84-16.5) as p< 0.05). Conclusion: The findings revealed the incidence of H. pylori infection (58%) and its associated risk factors in individuals having recurring epigastric pain. The results suggest that larger datasets are needed for future studies to accurately reflect infection frequency and risk factors to diagnose and treat the disease.
Zahoor Qadir Samra , School of Biochemistry and Biotechnology, The University of Punjab

(R) Director School of Biochemistry and Biotechnology
Aroosha Hussain, School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan

A number of stomach diseases, including gastritis, peptic ulcers, and gastric cancer, have been related to Helicobacter pylori (H. pylori), creating serious public health concerns. The current investigation aimed to determine the frequency of H. pylori infection and related risk factors in individuals having symptoms of recurring epigastric pain.Methods: The present study involves 191 participants having recurring epigastric pain. Sociodemographic data, blood, and tissue samples were obtained from 112 patients between September 2020 to August 2021. Biochemical and molecular methods were used to estimate the infection frequency.Results: The findings revealed that the overall infection ratio was 58% (n=65 out of 112). Males (n=41, 63.1%) contracted the infection more frequently than females. The participants belong to the 26-30 age group (n=37, 56.9%) low socioeconomic status (n=45, 69.2%), resided in rural areas (n=54, 83.1%), and consumed unfiltered water (n=57, 87.7%) had observed with significantly higher infection rates. The regression analysis demonstrated greater probability of contracting infection in male gender (OR 5.91, 95%CI: 2.40-12.9), in 26-35 age group (OR 6.16, 95%CI: 1.75-21.6), tap water users (OR 4.42, 95%CI: 1.72-11.3), rural area residents (OR 3.31, 95%CI:1.39-9.76), and low economic status group (OR 5.26, 95%CI:1.84-16.5) as p< 0.05). Conclusion: The findings revealed the incidence of H. pylori infection (58%) and its associated risk factors in individuals having recurring epigastric pain. The results suggest that larger datasets are needed for future studies to accurately reflect infection frequency and risk factors to diagnose and treat the disease.

References

Abadi AT, Kusters JG. Management of Helicobacter pylori infections. BMC gastroenterology. 2016;16(1):1-4.

Gu H. Role of Flagella in the Pathogenesis of Helicobacter pylori. Current microbiology. 2017;74:863-9.

Kusters JG, Van Vliet AH, Kuipers EJ. Pathogenesis of Helicobacter pylori infection. Clinical microbiology reviews. 2006;19(3):449-90.

Bazzoli F, De Luca L, Graham DY. Helicobacter pylori infection and the use of NSAIDs. Best Pract Res Clin Gastroenterol. 2001;15:775-785.

Mateos-Muñoz B, Pérez-de-la-Serna J, Ruiz-de-León A, Serrano-Falcón B, Casabona-Francés S, Velasco-Cerrudo A, Rey-Díaz-Rubio E. Enterohepatic Helicobacter other than Helicobacter pylori. Rev Esp Enferm Dig. 2013;105:477-484.

Peleteiro B, Bastos A, Ferro A, Lunet N. Prevalence of Helicobacter pylori infection worldwide: a systematic review of studies with national coverage. Dig Dis Sci. 2014;59:1698-709.

Suzuki H, Moayyedi P. Helicobacter pylori infection in functional dyspepsia. Nat Rev Gastroenterol Hepatol. 2013;10: 168-174.

Suzuki S, Kusano C, Horii T, Ichijima R, Ikehara H. The ideal Helicobacter pylori treatment for the present and the future. Digestion, (2022);103(1):62-8.

S Syahniar R, Kharisma DS. Helicobacter pylori Challenge Vaccine for Humans. InVaccine Development 2021. IntechOpen.

Roma E, Panayiotou J, Kafritsa Y, Van‐Vliet C, Gianoulia A, Constantopoulos A. Upper gastrointestinal disease, Helicobacter pylori and recurrent abdominal pain. Acta Pædiatrica. 1999;88: 598-601.

Gieteling MJ, Bierma-Zeinstra SM, van Leeuwen Y, Passchier J, Berger MY. Prognostic factors for persistence of chronic abdominal pain in children. J Pediatr Gastroenterol Nutr, (2011); 52: 154-161.

Rasool F, Siddique J, Anwar B. Prevalence of H. pylori Infection in the Children having Recurrent Abdominal Pain. Sys Rev Pharm. 2021;12:3391-3394.

Mansour M, Al Hadidi Kh M, Omar MA. Helicobacter pylori and recurrent abdominal pain in children: is there any relation? Trop Gastroenterol, (2012); 33: 55-61.

Czinn SJ. Helicobacter pylori infection: Detection, investigation, and management. J Pediatr. 2005;146:S21-S26.

Godavarthy PK, Puli C. From antibiotic resistance to antibiotic renaissance: a new era in Helicobacter pylori treatment. Cureus. 2023;15(3).

Shrivastava SR, Shrivastava PS, Ramasamy J. World health organization releases global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. Journal of Medical Society. 2018;32(1):76-7.

Fernández M, Contreras M, Suárez P, Gueneau P, García‐Amado MA. Use of HP selective medium to detect Helicobacter pylori associated with other enteric bacteria in seawater and marine molluscs. Lett Appl Microbiol. 2007; 45:213-218.

Taylor WI, Achanzar D. Catalase test as an aid to the identification of Enterobacteriaceae. Appl Microbiol. 1972;24:58-61.

Dahlén G, Hassan H, Blomqvist S, Carlén A. Rapid urease test (RUT) for evaluation of urease activity in oral bacteria in vitro and in supragingival dental plaque ex vivo. BMC Oral Health. 2018;18:1-7.

Al-Sulami AA, Al-Edani TA, Al-Abdula AA. Culture method and PCR for the detection of Helicobacter pylori in drinking water in Basrah Governorate Iraq. Gastroenterol Res Pract. 2012;2012:1-5.

Iqbal M, Philbin VJ, Smith AL. Expression patterns of chicken Toll-like receptor mRNA in tissues, immune cell subsets and cell lines. Vet Immunol Immunopathol. 2005;104:117-127.

Jaime F, Villagrán A, Hernández C, Ortiz M, Serrano C, Harris PR. Functional gastrointestinal disorders in children from low socio‐economic status and Helicobacter pylori infection. Child Care Health Dev. 2018;44:319-325.

Salih BA. Helicobacter pylori infection in developing countries: the burden for how long? Saudi J Gastroentero. 2009;15:201-207.

Öztekin M, Yılmaz B, Ağagündüz D, Capasso R. Overview of Helicobacter pylori Infection: Clinical Features, treatment, and nutritional aspects. Diseases. 2021;9:1-19.

Shah SRH, Almugadam BS, Hussain A, Ahmad T, Ahmed S, Sadiqui S. Epidemiology and risk factors of Helicobacter pylori infection in Timergara city of Pakistan: A cross-sectional study. Clin Epidemiol Glob Health. 2021;12:1-5.

Shokrzadeh L, Baghaei K, Yamaoka Y, Shiota S, Mirsattari D, Porhoseingholi A, Zali MR. Prevalence of Helicobacter pylori infection in dyspeptic patients in Iran. Gastroenterol insights. 2012;4:24-27.

Asiimwe D, Bangi I, Esanyu J, Ojok D, Okot B, Olong C, Wagubi R, Kisembo G, Sempijja F, Muwanguzi E, Okongo B. Association Between Helicobacter pylori Infection and Anemia Among Adult Dyspeptic Patients Attending Kiryandongo General Hospital, Uganda. Journal of Blood Medicine. 2023;31:57-66.

Wang W, Jiang W, Zhu S, Sun X, Li P, Liu K, Liu H, Gu J, Zhang S. Assessment of prevalence and risk factors of Helicobacter pylori infection in an oilfield Community in Hebei, China. BMC Gastroenterol. 2019;19:1-8.

Kouitcheu Mabeku LB, Noundjeu Ngamga ML, Leundji H. Potential risk factors and prevalence of Helicobacter pylori infection among adult patients with dyspepsia symptoms in Cameroon. BMC Infect Dis. 2018;18: 1-11.

Rastogi M, Rastogi D, Singh S, Agarwal A, Priyadarshi B, Middha T. Prevalence of Helicobacter pylori in asymptomatic adult patients in a tertiary care hospital: A cross sectional study. Biomed Res. 2015;26:117-122.

Calderon RL, Johnson Jr CC, Craun GF, Dufour AP, Karlin RJ, Sinks T, Valentine JL. Health risks from contaminated water: Do class and race matter? Toxicol Ind Health. 1993;9:879-900.

Luoto S, Krama T, Rubika A, Borráz-León JI, Trakimas G, Elferts D, Skrinda I, Krams R, Moore FR, Birbele E, Kaminska I. Socioeconomic position, immune function, and its physiological markers. Psychoneuroendocrinology. 2021;127:1-9.

de Martel C, Parsonnet J. Helicobacter pylori Infection and Gender: A Meta-Analysis of Population-Based Prevalence Surveys. Dig Dis Sci. 2006;51:2292–2301.

Nalbandian G, Kovats S. Understanding sex biases in immunity: Effects of estrogen on the differentiation and function of antigen-presenting cells. Immunol Res. 2005;31:91–106.

Morell V. Zeroing in on how hormones affect the immune system. Science. 1995;269:773–775.

Ismail MB, Osman M, Bou Raad E, Achkar M, Hamze M. Seroprevalence of Helicobacter pylori among dyspeptic patients in northern Lebanon: a 6-year retrospective study in two tertiary hospitals. Access Microbiology. 2022;4(4):000337.

Helicobacter Pylori Prevalence and Associated Risk Factors in Individuals with Recurrent Epigastric Pain: Lahore, Pakistan

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