Techniques for Monitoring Immune Responses in Animals Post Vaccination in the Era COVID-19

Main Article Content

Nasrullah
Nadia Jamil
Sheziab Saddiqui
Shahid Mahmood Chuhan
Waqar Hassan
Maliha Wajeeh
Zahra Zahid Piracha
Ali Dino
Kamran Khan
Muhammad Waqar Younas Abbasi
Muhammad Zohaib Khan
Saeed Khan
Rahat Bano
Saher Tariq
Muhammad Rashid
Kamran Ullah
Mujtaba Babar
Saifullah Khan

Abstract

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccines.

Article Details

How to Cite
Nasrullah, Nadia Jamil, Sheziab Saddiqui, Shahid Mahmood Chuhan, Waqar Hassan, Maliha Wajeeh, Zahra Zahid Piracha, Ali Dino, Khan, K., Muhammad Waqar Younas Abbasi, Muhammad Zohaib Khan, Saeed Khan, Rahat Bano, Saher Tariq, Muhammad Rashid, Kamran Ullah, Mujtaba Babar, & Saifullah Khan. (2023). Techniques for Monitoring Immune Responses in Animals Post Vaccination in the Era COVID-19. Pak-Euro Journal of Medical and Life Sciences, 6(2), 91–106. https://doi.org/10.31580/pjmls.v6i2.2605
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Articles
Author Biographies

Nasrullah, Center for Advanced Studies in Vaccinology and Biotechnology, (CASVAB), UoB, Quetta

DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccines.

Nadia Jamil, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Sheziab Saddiqui, Department of Biotechnology, Federal Urdu University of Arts Science and Technology, Karachi, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Shahid Mahmood Chuhan, Center for Excellence in Science and Applied Technology (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Waqar Hassan, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Maliha Wajeeh, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Zahra Zahid Piracha, Department of Microbiology, Ajou University, Suwon, South Korea

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Ali Dino, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Kamran Khan, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Muhammad Waqar Younas Abbasi, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Muhammad Zohaib Khan, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Saeed Khan, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Rahat Bano, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Saher Tariq, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Muhammad Rashid, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Kamran Ullah, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Mujtaba Babar, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

Saifullah Khan, Center for Excellence in Science and Applied Technologies (CESAT), Islamabad, Pakistan

The immune response is a critical aspect of an organism's defense against pathogens and diseases. Monitoring immune responses in animals is essential for understanding the mechanisms of immunity, developing vaccines, and evaluating the efficacy of immunotherapies. This review article discusses various techniques for monitoring immune responses including enzyme-linked immunosorbent assay (ELISA), Serum virus neutralization (SVN) test, Haemoagglutination (HI) test, flow cytometry, ELISpot assay, polymerase chain reaction (PCR), and imaging techniques for vaccine developed, the techniques monitoring both cellular and humoral immune response which are most important for vaccine machine understanding and mechanism. DNA vaccines have emerged as a promising approach in the field of immunization due to their ability to induce robust and long-lasting immune responses against a wide range of pathogens and diseases. Understanding and monitoring the immune responses elicited by DNA vaccines are crucial for assessing their efficacy, safety, and potential for clinical applications. This review article aims to provide a comprehensive overview of the various techniques used for monitoring immune responses against DNA vaccines, with a focus on key immunological parameters, experimental methodologies, and recent advancements. Additionally, we discuss the significance of these monitoring techniques in advancing the development and optimization of DNA vaccine<em>s.</em>

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