In Vitro and In Vivo Anti-Inflammatory Potential of Octhochloa Compressa Extracts in Carrageenan Induced Rats
DOI:
https://doi.org/10.31580/pjmls.v4i4.1717Keywords:
Salmonellosis, Salmonella, Ready to Eat Food, Food Contamination, Conventional methods for microbial detection, Biochemical TestsAbstract
Octhochloa compressa (Poaceae) is a desert plant of Cholistan traditionally used as a food source by local nomads. Different extracts of Octhochloa compressa: aqueous (Aqu), methanol (MetOH), n-butanol (n-But), ethyl acetate (EtAc), n-hexane (n-Hex) and dichloromethane (DCM)) were examined for anti-inflammatory activity by in vitro human red blood cell (HRBC) membrane stabilization assay and invivo carrageenaninduced oedema in rat paw. The potency of O. compressa extracts was compared with diclofenac at 15mg/ml and 10mg/kg for invivoand invitrotrials, respectively. According to anti-inflammatory activity in the in vitro experiment, MetOH extract was exhibited the remarkable potential among all extracts with 85% protection of HRBC lysis (IC50 231.7±0.678µg/ml; p<0.001), even better than standard drug (diclofenac). Therefore, the most promising and effective MetOH and EtAc extracts in the in vitro experiments were selected for further testing of in vivo anti-inflammatory activity. In the in vivo assay, among three doses of MetOH extracts, 300mg/kg BW dose of MetOH extract shown highly significant (p<0.001) reduction in the carrageenan induced paw oedema (from 8.48±0.20mm at 1h to 7.67±0.44mm at 4h) compared to diclofenac (from 8.13±0.55mm at 1h to 7.48±0.21mm at 4h). Hence, MetOH extract showed the most promising anti-inflammatory activity in the membrane stabilizing action on HRBC membrane and reduction of oedema in carrageenan induced rat paw model. Hence, the plant could be recommended as a therapeutic agent against the inflammatory diseases.
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