Synthesis of Silver Nanoparticles and Valuation of Their Antibacterial Activity Against Gram Positive & Gram-Negative Bacteria


Silver nanoparticles
antimicrobial activity
E. coli
Staphylococcus aureus
Bacillus subtilis
Klebsiella pneumonia
Pseudomonas aeruginosa

How to Cite

Malik, R. R., Sabeera Afzal, Muhammad Bilal, Yasir, & AsadUllah. (2020). Synthesis of Silver Nanoparticles and Valuation of Their Antibacterial Activity Against Gram Positive & Gram-Negative Bacteria. Pak-Euro Journal of Medical and Life Sciences, 3(3), 113-120.


Background:Nanoparticles are involved in multiple applications of our everydaylives as well as a myriad of diagnostic and therapeutic uses.Nanoparticles have greater surface area to volume ratio, hence greater penetrability. This allows for both their efficacy and toxicity.From drug delivery systems to sun screens to imagingtechniques, nanoparticles are part of various aspects of modernlife. On the basis of the properties of silver, nanotechnology is now using silver in the form of silver nanoparticles. Silver nanoparticles hold great importance because several pathogenic bacteria have developed resistance against the antibiotics which were used for treating bacterial infections. Objective:The aim of this study was the Synthesis of Silver Nanoparticles and Valuation of their Antibacterial Activity against Gram Positive & Gram-Negative Bacteria. Methodology:Silver nanoparticles were prepared from E coli then were coated on the different antibiotics to enhance their antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumonia, Pseudomonas aeruginosa. Results: After coating the antibiotics with silver nanoparticles, the antibacterial activity of antibiotics against their respective bacterium increases. Conclusion: Moreover, comparison between gram positive & gram-negative bacteria against nanoparticle coated antibiotics was also studied which shows thateffect of silver nanoparticles is more enhanced in case of gram-positive bacteria as compared to that of gram-negative bacteria.


Adimpong, D. B., Sørensen, K. I., Thorsen, L., Stuer-Lauridsen, B., Abdelgadir, W. S., Nielsen, D. S., . . . Jespersen, L. (2012). Antimicrobial susceptibility of Bacillus strains isolated from primary starters for African traditional bread production and characterization of the bacitracin operon and bacitracin biosynthesis. Applied and environmental microbiology, 78(22), 7903-7914.
Aillon, K. L., Xie, Y., El-Gendy, N., Berkland, C. J., & Forrest, M. L. (2009). Effects of nanomaterial physicochemical properties on in vivo toxicity. Advanced drug delivery reviews, 61(6), 457-466.
El Bouamri, M., Arsalane, L., El Kamouni, Y., & Zouhair, S. (2015). Antimicrobial susceptibility of urinary Klebsiella pneumoniae and the emergence of carbapenem-resistant strains: A retrospective study from a university hospital in Morocco, North Africa. African Journal of Urology, 21(1), 36-40.
Emerich, D. F., & Thanos, C. G. (2003). Nanotechnology and medicine. Expert Opinion on Biological Therapy, 3(4), 655-663.
Gandhi, H., & Khan, S. (2016). Biological Synthesis of Silver Nanoparticles and Its Antibacterial Activity. Journal of Nanomedicine and Nanotechnology, 7(2), 1000366.
Geoprincy, G., Srri, B. V., Poonguzhali, U., Gandhi, N. N., & Renganathan, S. (2013). A review on green synthesis of silver nanoparticles. Asian Journal of Pharmaceutical and clinical research, 6(1), 8-12.
Kim, S.-H., Lee, H.-S., Ryu, D.-S., Choi, S.-J., & Lee, D.-S. (2011). Antibacterial activity of silver-nanoparticles against Staphylococcus aureus and Escherichia coli. Korean J. Microbiol. Biotechnol, 39(1), 77-85.
Kushwaha, A., Singh, V. K., Bhartariya, J., Singh, P., & Yasmeen, K. (2015). Isolation and identification of E. coli bacteria for the synthesis of silver nanoparticles: characterization of the particles and study of antibacterial activity. Eur J Exp Biol, 5(1), 65-70.
Monedeiro, F., Pomastowski, P., Milanowski, M., Ligor, T., & Buszewski, B. (2019). Monitoring of Bactericidal Effects of Silver Nanoparticles Based on Protein Signatures and VOC Emissions from Escherichia coli and Selected Salivary Bacteria. Journal of clinical medicine, 8(11), 2024.
Mourdikoudis, S., Pallares, R. M., & Thanh, N. T. (2018). Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties. Nanoscale, 10(27), 12871-12934.
Rai, M., Yadav, A., & Gade, A. (2009). Silver nanoparticles as a new generation of antimicrobials. Biotechnology advances, 27(1), 76-83.
Shahverdi, A. R., Fakhimi, A., Shahverdi, H. R., & Minaian, S. (2007). Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. Nanomedicine: Nanotechnology, Biology and Medicine, 3(2), 168-171.
Silva, G. A. (2004). Introduction to nanotechnology and its applications to medicine. Surgical neurology, 61(3), 216-220.
Stein, M., Komerska, J., Prizade, M., Sheinberg, B., Tasher, D., & Somekh, E. (2016). Clindamycin resistance among Staphylococcus aureus strains in Israel: implications for empirical treatment of skin and soft tissue infections. International Journal of Infectious Diseases, 46, 18-21.
Tran, Q. H., & Le, A.-T. (2013). Silver nanoparticles: synthesis, properties, toxicology, applications and perspectives. Advances in Natural Sciences: Nanoscience and Nanotechnology, 4(3), 033001.
Truong, V., Kumar, S. R., Pang, J.-H. S., Liu, Y.-K., Chen, D. W., & Lue, S. J. (2020). Synergistic antibacterial activity of silver-loaded graphene oxide towards Staphylococcus aureus and Escherichia coli. Nanomaterials, 10(2), 366.
Yayan, J., Ghebremedhin, B., & Rasche, K. (2015). Antibiotic resistance of Pseudomonas aeruginosa in pneumonia at a single university hospital center in Germany over a 10-year period. Plos one, 10(10), e0139836.
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.