Background:Nanoparticles are involved in multiple applications of our everydaylives as well as a myriad of diagnostic and therapeutic uses.Nanoparticles have greater surface area to volume ratio, hence greater penetrability. This allows for both their efficacy and toxicity.From drug delivery systems to sun screens to imagingtechniques, nanoparticles are part of various aspects of modernlife. On the basis of the properties of silver, nanotechnology is now using silver in the form of silver nanoparticles. Silver nanoparticles hold great importance because several pathogenic bacteria have developed resistance against the antibiotics which were used for treating bacterial infections. Objective:The aim of this study was the Synthesis of Silver Nanoparticles and Valuation of their Antibacterial Activity against Gram Positive & Gram-Negative Bacteria. Methodology:Silver nanoparticles were prepared from E coli then were coated on the different antibiotics to enhance their antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumonia, Pseudomonas aeruginosa. Results: After coating the antibiotics with silver nanoparticles, the antibacterial activity of antibiotics against their respective bacterium increases. Conclusion: Moreover, comparison between gram positive & gram-negative bacteria against nanoparticle coated antibiotics was also studied which shows thateffect of silver nanoparticles is more enhanced in case of gram-positive bacteria as compared to that of gram-negative bacteria.
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